GanDouLing Combined with Penicillamine Improves Cerebrovascular Injury Via PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in the Mouse Model of Wilson's Disease

Overview

Journal Biosci Rep

Specialty Cell Biology

Date 2018 Sep 6

PMID 30181379

Citations 6

Authors

Yonghua Chen

Bo Zhang

Shijian Cao

Wei Huang

Ni Liu

Wenming Yang

Affiliations

Soon will be listed here.

Abstract

We aim to investigate the function and mechanism of GanDouLing combinated with Penicillamine on cerebrovascular injury in Wilson's disease (WD). ELISA was performed to analyze the expression of vascular injury factors. Pathological changes of cerebral vessels were observed by HE stain. Immunohistochemistry assays were performed to analyze the expression of ICAM-1, VCAM-1, and GRP78. Western blotting was measured to analyze the expression of caspase-3, caspase-12, PERK, eIF2α, and CHOP. Apoptosis was detected with TUNEL assay. The expression of vascular injury factors and ICAM-1, VCAM-1 was significantly increased by WD and markedly decreased in GanDouLing-Penicillamine group. The expression of caspase-3, caspase-12, PERK, eIF2α, and CHOP were obviously expressed in Wilson group, GanDouLing-Penicillamine suppressed apoptosis and endoplasmic reticulum (ER) stress. Our findings suggested that GanDouLing-Penicillamine improved cerebrovascular injury through PERK/eIF2α/CHOP ER stress pathway in the mouse model of WD.

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