Osseointegrative Effect of RhBMP-2 Covalently Bound on a Titan-plasma-spray-surface After Modification with Chromosulfuric Acid in a Large Animal Bone Gap-healing Model with the Göttingen Minipig

Overview

Journal J Orthop Surg Res

Publisher Biomed Central

Specialty Orthopedics

Date 2018 Sep 1

PMID 30165865

Citations 1

Authors

Manuel Lingner

Roland Seidling

Lars Johannes Lehmann

Eckhard Mauermann

Udo Obertacke

Markus Ludwig Rupert Schwarz

Affiliations

Soon will be listed here.

Abstract

Background: Bone morphogenetic proteins play an important role as osseointegrative factors. It is used widely in orthopedic research and surgery to enhance the osseointegrative potential of implants, e.g., in spinal fusion or alveolar socket augmentation. The aim of the present study was to investigate the benefit of rhBMP-2 on a titan plasma spray (TPS) layer after a special modification with chromosulfuric acid (CSA) at different postoperative times, regarding osseoconduction and osseoinduction.

Methods: We allocated 27 Göttinger minipigs into three groups consisting of nine animals each. They received four dumbbell-shaped implants in the metaphyseal parts of the femora. The implants had a TPS surface with (CSA group) and without a CSA treatment (TPS group). The former received an additional layer of BMP-2 (BMP-2 group). For the assessment of osseointegration after healing periods of 4, 8, and 12 weeks, histomorphometry was applied to undecalcified specimens after staining according to Masson-Goldner. An intravital labeling with different fluorochromes was used in the gap model. A multivariable analysis with repeated measurement design was performed for statistical evaluation.

Results: We observed several statistical differences in a three-way ANOVA. The comparison between the BMP-2 and the TPS group (two-way ANOVA) showed statistically significant differences in terms of the osseoinduction (osteoid volume), and pronounced for the osseoconduction (bone and osteoid ongrowth), in favor of the BMP-2 group. In the pairwise comparison between BMP-2 and CSA (two-way ANOVA), no statistical significance occurred. The intravital staining with tetracycline, calcein green, and xylenol orange revealed no considerable differences between the groups.

Conclusion: BMP-2, covalently bound on a CSA-treated TPS surface, has positive effects on the osseointegration in the large animal bone gap-healing model over the observation period of 12 weeks.

Citing Articles

Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development.

Gonzalez Lopez M, Huteckova B, Lavicky J, Zezula N, Rakultsev V, Fridrichova V Sci Adv. 2023; 9(31):eadi0482.

PMID: 37531427 PMC: 10396306. DOI: 10.1126/sciadv.adi0482.

References

Schmalzried T, Jasty M, Harris W . Periprosthetic bone loss in total hip arthroplasty. Polyethylene wear debris and the concept of the effective joint space. J Bone Joint Surg Am. 1992; 74(6):849-63. View

Wolfle J, Fiedler J, Durselen L, Reichert J, Scharnweber D, Forster A . Improved anchorage of Ti6Al4V orthopaedic bone implants through oligonucleotide mediated immobilization of BMP-2 in osteoporotic rats. PLoS One. 2014; 9(1):e86151. PMC: 3897651. DOI: 10.1371/journal.pone.0086151. View

Christensen B, Foldager C, Olesen M, Vingtoft L, Rolfing J, Ringgaard S . Experimental articular cartilage repair in the Göttingen minipig: the influence of multiple defects per knee. J Exp Orthop. 2016; 2(1):13. PMC: 4538720. DOI: 10.1186/s40634-015-0031-3. View

De Biase P, Capanna R . Clinical applications of BMPs. Injury. 2005; 36 Suppl 3:S43-6. DOI: 10.1016/j.injury.2005.07.034. View

Urist M . Bone: formation by autoinduction. Science. 1965; 150(3698):893-9. DOI: 10.1126/science.150.3698.893. View

Lehmann L, Werner A, Dinter D, Mauermann E, Seidling R, Brade J . Scintigraphic evaluation of rhBMP-2-biocoated implants reveals no ectopic bone formation. Biomed Pharmacother. 2010; 65(1):63-8. DOI: 10.1016/j.biopha.2010.10.008. View

Rahbek O, Overgaard S, Jensen T, Bendix K, Soballe K . Sealing effect of hydroxyapatite coating: a 12-month study in canines. Acta Orthop Scand. 2001; 71(6):563-73. DOI: 10.1080/000164700317362181 . View

Draenert Y, Draenert K . Gap healing of compact bone. Scan Electron Microsc. 1980; (4):103-11. View

Raida M, Heymann A, Gunther C, Niederwieser D . Role of bone morphogenetic protein 2 in the crosstalk between endothelial progenitor cells and mesenchymal stem cells. Int J Mol Med. 2006; 18(4):735-9. DOI: 10.3892/ijmm.18.4.735. View

10.

Aerssens J, Boonen S, Lowet G, Dequeker J . Interspecies differences in bone composition, density, and quality: potential implications for in vivo bone research. Endocrinology. 1998; 139(2):663-70. DOI: 10.1210/endo.139.2.5751. View

11.

Dimitriou R, Tsiridis E, Giannoudis P . Current concepts of molecular aspects of bone healing. Injury. 2005; 36(12):1392-404. DOI: 10.1016/j.injury.2005.07.019. View

12.

Wagner A, Sachse A, Keller M, Aurich M, Wetzel W, Hortschansky P . Qualitative evaluation of titanium implant integration into bone by diffraction enhanced imaging. Phys Med Biol. 2006; 51(5):1313-24. DOI: 10.1088/0031-9155/51/5/019. View

13.

Heino T, Alm J, Moritz N, Aro H . Comparison of the osteogenic capacity of minipig and human bone marrow-derived mesenchymal stem cells. J Orthop Res. 2012; 30(7):1019-25. DOI: 10.1002/jor.22049. View

14.

Pautke C, Vogt S, Tischer T, Wexel G, Deppe H, Milz S . Polychrome labeling of bone with seven different fluorochromes: enhancing fluorochrome discrimination by spectral image analysis. Bone. 2005; 37(4):441-5. DOI: 10.1016/j.bone.2005.05.008. View

15.

Lind M . Growth factor stimulation of bone healing. Effects on osteoblasts, osteomies, and implants fixation. Acta Orthop Scand Suppl. 1998; 283:2-37. View

16.

Parfitt A, Drezner M, Glorieux F, Kanis J, MALLUCHE H, Meunier P . Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. 1987; 2(6):595-610. DOI: 10.1002/jbmr.5650020617. View

17.

OLERUD S, LORENZI G . Triple fluorochrome labeling in bone formation and bone resorption. J Bone Joint Surg Am. 1970; 52(2):274-8. View

18.

Anderson C, Whitaker M . Heterotopic ossification associated with recombinant human bone morphogenetic protein-2 (infuse) in posterolateral lumbar spine fusion: a case report. Spine (Phila Pa 1976). 2011; 37(8):E502-6. DOI: 10.1097/BRS.0b013e318238870b. View

19.

Christensen B . Autologous tissue transplantations for osteochondral repair. Dan Med J. 2016; 63(4). View

20.

Donath K, Breuner G . A method for the study of undecalcified bones and teeth with attached soft tissues. The Säge-Schliff (sawing and grinding) technique. J Oral Pathol. 1982; 11(4):318-26. DOI: 10.1111/j.1600-0714.1982.tb00172.x. View