The Rab7 Subfamily Across Species; Evidence of High Conservation in Sequence and Function

Overview

Journal Small GTPases

Specialties Biochemistry
Cell Biology

Date 2018 Aug 30

PMID 30156960

Authors

Lydia J Bright

Michael Lynch

Affiliations

Soon will be listed here.

Abstract

We examined sequence conservation and signatures of selection in Rab7 proteins across 11 species, and determined the localization patterns of two Rab7 paralogs when expressed as GFP fusions in live cells. We found that, while there is a variable number of Rab7 paralogs per genome, Rab7 genes are highly conserved in sequence and appear to be under strong purifying selection across . Additionally, and surprisingly based on earlier studies, we found that two Rab7 proteins have virtually identical localization patterns. Consistent with this, when we examined the gene family of a highly conserved Rab binding partner across (Rab-Interacting Lysosomal Protein, or RILP), we found that residues in key binding sites in RILPs were absolutely conserved in 13 of 21 proteins, representing genes from 9 of the 11 species examined. Of note, RILP gene number appears to be even more constrained than Rab7 gene number per genome. : WGD: Whole genome duplication.

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