Intraspinal Grafting of Serotonergic Neurons Modifies Expression of Genes Important for Functional Recovery in Paraplegic Rats

Overview

Journal Neural Plast

Specialty Neurology

Date 2018 Aug 28

PMID 30147717

Citations 4

Authors

Krzysztof Miazga

Hanna Fabczak

Ewa Joachimiak

Malgorzata Zawadzka

Lucja Krzemien-Ojak

Marek Bekisz

Anna Bejrowska

Larry M Jordan

Urszula Slawinska

Affiliations

Soon will be listed here.

Abstract

Serotonin (5-hydroxytryptamine; 5-HT) plays an important role in control of locomotion, partly through direct effects on motoneurons. Spinal cord complete transection (SCI) results in changes in 5-HT receptors on motoneurons that influence functional recovery. Activation of 5-HT and 5-HT receptors improves locomotor hindlimb movements in paraplegic rats. Here, we analyzed the mRNA of 5-HT and 5-HT receptors (encoded by and genes, resp.) in motoneurons innervating tibialis anterior (TA) and gastrocnemius lateralis (GM) hindlimb muscles and the tail extensor caudae medialis (ECM) muscle in intact as well as spinal rats. Moreover, the effect of intraspinal grafting of serotonergic neurons on and gene expression was examined to test the possibility that the graft origin 5-HT innervation in the spinal cord of paraplegic rats could reverse changes in gene expression induced by SCI. Our results indicate that SCI at the thoracic level leads to changes in and gene expression, whereas transplantation of embryonic serotonergic neurons modifies these changes in motoneurons innervating hindlimb muscles but not those innervating tail muscles. This suggests that the upregulation of genes critical for locomotor recovery, resulting in limb motoneuron plasticity, might account for the improved locomotion in grafted animals.

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