Localization and DNA Sequence Analysis of the C Gene of Bacteriophage Mu, the Positive Regulator of Mu Late Transcription

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Jun 25

PMID 3014438

Citations 24

Authors

W Margolin

M M Howe

Affiliations

Soon will be listed here.

Abstract

The C gene of bacteriophage Mu, required for transcription of the phage late genes, was localized by construction and analysis of a series of deleted derivatives of pKN50, a plasmid containing a 9.4 kb Mu DNA fragment which complements Mu C amber mutant phages for growth. One such deleted derivative, pWM10, containing only 0.5 kb of Mu DNA, complements C amber phages and transactivates the mom gene, one of the Mu late genes dependent on C for activation. The DNA sequence of the 0.5 kb fragment predicts a single long open reading frame coding for a 140 amino acid protein. Sequence analysis of DNA containing a C amber mutation located the base change to the second codon of this reading frame. Generation of a frameshift mutation by filling in a BglII site spanning codon 114 of this reading frame resulted in the loss of C complementation and transactivation activity. These results indicate that this open reading frame encodes the Mu C gene product. Comparison of the predicted amino acid sequence of the C protein with those of other transcriptional regulatory proteins revealed some similarity to a region highly conserved among bacterial sigma factors.

Citing Articles

Different Modes of Transactivation of Bacteriophage Mu Late Promoters by Transcription Factor C.

Swapna G, Kumari V, Nagaraja V PLoS One. 2015; 10(6):e0129504.

PMID: 26058069 PMC: 4461284. DOI: 10.1371/journal.pone.0129504.

Genetic analysis of phage Mu Mor protein amino acids involved in DNA minor groove binding and conformational changes.

Kumaraswami M, Avanigadda L, Rai R, Park H, Howe M J Biol Chem. 2011; 286(41):35852-35862.

PMID: 21859715 PMC: 3195604. DOI: 10.1074/jbc.M111.269860.

RinA controls phage-mediated packaging and transfer of virulence genes in Gram-positive bacteria.

Ferrer M, Quiles-Puchalt N, Harwich M, Tormo-Mas M, Campoy S, Barbe J Nucleic Acids Res. 2011; 39(14):5866-78.

PMID: 21450808 PMC: 3152322. DOI: 10.1093/nar/gkr158.

Interaction between bacteriophage DMS3 and host CRISPR region inhibits group behaviors of Pseudomonas aeruginosa.

Zegans M, Wagner J, Cady K, Murphy D, Hammond J, OToole G J Bacteriol. 2008; 191(1):210-9.

PMID: 18952788 PMC: 2612449. DOI: 10.1128/JB.00797-08.

Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding.

Jiang Y, Howe M Nucleic Acids Res. 2008; 36(20):6396-405.

PMID: 18838393 PMC: 2582627. DOI: 10.1093/nar/gkn639.

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