Structural Conservation in a Membrane-enveloped Filamentous Virus Infecting a Hyperthermophilic Acidophile

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Aug 24

PMID 30135568

Citations 20

Authors

Ying Liu

Tomasz Osinski

Fengbin Wang

Mart Krupovic

Stefan Schouten

Peter Kasson

David Prangishvili

Edward H Egelman

Affiliations

Soon will be listed here.

Abstract

Different forms of viruses that infect archaea inhabiting extreme environments continue to be discovered at a surprising rate, suggesting that the current sampling of these viruses is sparse. We describe here Sulfolobus filamentous virus 1 (SFV1), a membrane-enveloped virus infecting Sulfolobus shibatae. The virus encodes two major coat proteins which display no apparent sequence similarity with each other or with any other proteins in databases. We have used cryo-electron microscopy at 3.7 Å resolution to show that these two proteins form a nearly symmetrical heterodimer, which wraps around A-form DNA, similar to what has been shown for SIRV2 and AFV1, two other archaeal filamentous viruses. The thin (∼ 20 Å) membrane of SFV1 is mainly archaeol, a lipid species that accounts for only 1% of the host lipids. Our results show how relatively conserved structural features can be maintained across evolution by both proteins and lipids that have diverged considerably.

Citing Articles

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