Erythrocyte Arginase, Pyrimidine 5'-nucleotidase (P5N), and Deoxypyrimidine 5'-nucleotidase (dP5N) As Indices of Lead Exposure

Overview

Journal Br J Ind Med

Specialty Occupational Medicine

Date 1986 Jun 1

PMID 3013277

Citations 4

Authors

L R COOK

C R ANGLE

S J Stohs

Affiliations

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Abstract

The activities of three erythrocyte (rbc) enzymes, arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N), were compared in 16 lead workers and 14 age matched controls as correlates of blood lead (PbB) and unextracted zinc protoporphyrin (EP) concentrations. Subjects with PbB of 0.9-2.5 microM (19-52 micrograms/dl) had 6.5 +/- 0.6 IU of P5N activity with uridine monophosphate (UMP) as substrate, significantly less (p less than 0.001) than the 12.0 +/- 0.7 IU activity of controls with PbB 0.3-0.6 microM (6-12 micrograms/dl). The mean activity of rbc dP5N with either deoxyuridine monophosphate (dUMP) or thymidine monophosphate as substrate, and of rbc arginase, did not differentiate the two groups. The correlation coefficients of ln PbB with the selected substrates for P5N and dP5N were: UMP, r = -0.75; dUMP, r = -0.61; TMP, r = -0.23. The correlation coefficient of ln PbB and arginase activity was -0.03. Rbc P5N (UMPase) is a significant correlate of PbB, equivalent to rbc protoporphyrin. HPLC assay of rbc UMPase activity is a sensitive and rapid assay that appears to meet criteria for a reliable clinical laboratory index of blood lead concentrations.

Citing Articles

Relationship between inhibition of erythrocyte pyrimidine 5'-nucleotidase activity and biological response for porphyrin metabolism in workers occupationally exposed to lead.

Tomokuni K, Ichiba M, Hirai Y, Hasegawa T, Sugimoto K Int Arch Occup Environ Health. 1988; 60(6):431-6.

PMID: 2842263 DOI: 10.1007/BF00381391.

Determination of pyrimidine 5'-nucleotidase (P5N) activity in whole blood as an index of lead exposure.

Sakai T, Araki T, Ushio K Br J Ind Med. 1988; 45(6):420-5.

PMID: 2840110 PMC: 1009621. DOI: 10.1136/oem.45.6.420.

Studies on erythrocyte pyrimidine 5'-nucleotidase (P5N) test and its evaluation in workers occupationally exposed to lead.

Ichiba M, Tomokuni K Int Arch Occup Environ Health. 1990; 62(4):305-10.

PMID: 2379961 DOI: 10.1007/BF00640838.

Effects of lead on polymorphonuclear leukocyte (PMN) functions in occupationally exposed workers.

Valentino M, Governa M, Marchiseppe I, Visona I Arch Toxicol. 1991; 65(8):685-8.

PMID: 1660709 DOI: 10.1007/BF02098038.

References

Beutler E, West C, Blume K . The removal of leukocytes and platelets from whole blood. J Lab Clin Med. 1976; 88(2):328-33. View

Paglia D, Valentine W, Fink K . Lead poisoning. Further observations on erythrocyte pyrimidine-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotides. J Clin Invest. 1977; 60(6):1362-6. PMC: 372493. DOI: 10.1172/JCI108896. View

Buc H, Kaplan J . Red-cell pyrimidine 5'-nucleotidase and lead poisoning. Clin Chim Acta. 1978; 87(1):49-55. DOI: 10.1016/0009-8981(78)90056-6. View

Orfanos A, Naylor E, Guthrie R . Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper. Clin Chem. 1980; 26(8):1198-200. View

Chmielnicka J, Komsta-Szumska E, Szymanska J . Arginase and kallikrein activities as biochemical indices of occupational exposure to lead. Br J Ind Med. 1981; 38(2):175-8. PMC: 1008842. DOI: 10.1136/oem.38.2.175. View

Tomokuni K, Ogata M . Relationship between lead concentration in blood and the activities of erythrocyte pyrimidine 5'-nucleotidase and delta-aminolevulinate dehydratase in the mice exposed to lead. Arch Toxicol. 1981; 47(1):67-9. DOI: 10.1007/BF00297133. View

ANGLE C, McINTIRE M . Low level lead and inhibition of erythrocyte pyrimidine nucleotidase. Environ Res. 1978; 17(2):296-302. DOI: 10.1016/0013-9351(78)90032-4. View

Fukumoto K, Karai I, Nishikawa Y, Horiguchi S . Erythrocyte arginase activity as an indicator of lead exposure. Br J Ind Med. 1983; 40(1):106-10. PMC: 1009127. DOI: 10.1136/oem.40.1.106. View

Buchet J, Bernard A, Lauwerys R . In vitro effects of lead, mercury and cadmium on the enzymic activity of red-blood cell pyrimidine 5'-nucleotidase. Toxicol Lett. 1984; 20(2):195-9. DOI: 10.1016/0378-4274(84)90147-4. View

10.

Comoy E, Bohuon C, Boudene C . Immunoturbidimetric assay of delta aminolevulinic acid dehydratase (ALA D) on a centrifugal fast analyser. Clin Chim Acta. 1985; 147(2):179-81. DOI: 10.1016/0009-8981(85)90080-4. View

11.

TORRANCE J, Mills W, KILROE-SMITH T, Smith A . Erythrocyte pyrimidine-5'-nucleotidase activity as a sensitive indicator of lead exposure. S Afr Med J. 1985; 67(21):850-2. View

12.

Cook L, Angle C, Stohs S . Assay of human erythrocyte pyrimidine and deoxypyrimidine 5'-nucleotidase by isocratic reversed-phase high-performance liquid chromatography. J Chromatogr. 1985; 339(2):293-301. DOI: 10.1016/s0378-4347(00)84656-3. View

13.

Rocco R, Smart J, Brown D, Blumberg J . HPLC analysis of erythrocyte pyrimidine-5'-nucleotidase inhibition by lead. Res Commun Chem Pathol Pharmacol. 1981; 34(1):127-36. View