Environmentally-Friendly Extraction of Flavonoids from (Batal.) Iljinskaja Leaves with Deep Eutectic Solvents and Evaluation of Their Antioxidant Activities

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Aug 23

PMID 30131481

Citations 13

Authors

Xianchao Shang

Jia-Neng Tan

Yongmei Du

Xinmin Liu

Zhongfeng Zhang

Affiliations

Soon will be listed here.

Abstract

Deep eutectic solvents (DESs) are commonly employed as environmentally-friendly solvents in numerous chemical applications owing to their unique physicochemical properties. In this study, a novel and environmentally-friendly extraction method based on ultrasound assisted-deep eutectic solvent extraction (UAE-DES) was investigated for the extraction of flavonoids from (Batal.) Iljinskaja () leaves, and the antioxidant activities of these flavonoids were evaluated. Nine different DES systems based on either two or three components were tested, and the choline chloride/1,4⁻butanediol system (1:5 molar ratio) was selected as the optimal system for maximizing the flavonoid extraction yields. Other extraction conditions required to achieve the maximum flavonoid extraction yields from the leaves of were as follows: DES water content (/), 30%; extraction time, 30 min; temperature, 60 °C; and solid-liquid ratio, 20 mg/mL. Liquid chromatography-mass spectrometry allowed the detection of five flavonoids in the extract, namely kaempferol-7--α-l-rhamnoside, kaempferol, quercetin, quercetin-3--β-d-glucuronide, and kaempferol-3--β-d-glucuronide. In vitro antioxidant tests revealed that the flavonoid-containing extract exhibited strong DPPH and ABTS radical-scavenging abilities. Results indicate that UAE-DES is a suitable approach for the selective extraction of flavonoids from leaves, and DESs can be employed as sustainable extraction media for other bioactive compounds.

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