Roles of 5-HT on Phase Transition of Neurite Outgrowth in the Identified Serotoninergic Neuron C1, Helisoma Trivolvis

Overview

Journal Invert Neurosci

Date 2018 Aug 22

PMID 30128715

Authors

Kee-Chan Ahn

Glen B Baker

Won-Cheoul Jang

Hyeon-Cheol Cha

Myung Jin Moon

Mee-Sook Song

Affiliations

Soon will be listed here.

Abstract

Neurite outgrowth is a morphological marker of neuronal differentiation and neuroregeneration, and the process includes four essential phases, namely initiation, elongation, guidance and cessation. Intrinsic and extrinsic signaling molecules seem to involve morphological changes of neurite outgrowth via various cellular signaling cascades phase transition. Although mechanisms associated with neurite outgrowth have been studied extensively, little is known about how phase transition is regulated during neurite outgrowth. 5-HT has long been studied with regard to its relationship to neurite outgrowth in invertebrate and vertebrate culture systems, and many studies have suggested 5-HT inhibits neurite elongation and growth cone motility, in particular, at the growing parts of neurite such as growth cones and filopodia. However, the underlying mechanisms need to be investigated. In this study, we investigated roles of 5-HT on neurite outgrowth using single serotonergic neurons C1 isolated from Helisoma trivolvis. We observed that 5-HT delayed phase transitions from initiation to elongation of neurite outgrowth. This study for the first time demonstrated that 5-HT has a critical role in phase-controlling mechanisms of neurite outgrowth in neuronal cell cultures.

References

Tajima H, Sunaga K, Tanaka M, Kuwae T, Katsube N . Overexpression of glyceraldehyde-3-phospahe dehydrogenase is not involved in 5-hydroxytryptamine (5-HT)-induced necrosis in cultured cerebrocortical neurons. Biol Pharm Bull. 2004; 27(8):1224-7. DOI: 10.1248/bpb.27.1224. View

Dehmelt L, Halpain S . Actin and microtubules in neurite initiation: are MAPs the missing link?. J Neurobiol. 2003; 58(1):18-33. DOI: 10.1002/neu.10284. View

McKerracher L, Winton M . Nogo on the go. Neuron. 2002; 36(3):345-8. DOI: 10.1016/s0896-6273(02)01018-8. View

Zhang X, Hyland C, Van Goor D, Forscher P . Calcineurin-dependent cofilin activation and increased retrograde actin flow drive 5-HT-dependent neurite outgrowth in Aplysia bag cell neurons. Mol Biol Cell. 2012; 23(24):4833-48. PMC: 3521690. DOI: 10.1091/mbc.E12-10-0715. View

Lieske V, Bennett-Clarke C, Rhoades R . Effects of serotonin on neurite outgrowth from thalamic neurons in vitro. Neuroscience. 1999; 90(3):967-74. DOI: 10.1016/s0306-4522(98)00501-6. View

Jequier E, Lovenberg W, SJOERDSMA A . Tryptophan hydroxylase inhibition: the mechanism by which p-chlorophenylalanine depletes rat brain serotonin. Mol Pharmacol. 1967; 3(3):274-8. View

Duhr F, Deleris P, Raynaud F, Seveno M, Morisset-Lopez S, Mannoury la Cour C . Cdk5 induces constitutive activation of 5-HT6 receptors to promote neurite growth. Nat Chem Biol. 2014; 10(7):590-7. DOI: 10.1038/nchembio.1547. View

Murphy A, Barker D, Loring J, Kater S . Sprouting and functional regeneration of an identified serotonergic neuron following axotomy. J Neurobiol. 1985; 16(2):137-51. DOI: 10.1002/neu.480160206. View

Truman J, de Vente J, Ball E . Nitric oxide-sensitive guanylate cyclase activity is associated with the maturational phase of neuronal development in insects. Development. 1996; 122(12):3949-58. DOI: 10.1242/dev.122.12.3949. View

10.

Weissmann D, Belin M, Aguera M, Meunier C, Maitre M, CASH C . Immunohistochemistry of tryptophan hydroxylase in the rat brain. Neuroscience. 1987; 23(1):291-304. DOI: 10.1016/0306-4522(87)90290-9. View

11.

Jequier E, Robinson D, Lovenberg W, SJOERDSMA A . Further studies on tryptophan hydroxylase in rat brainstem and beef pineal. Biochem Pharmacol. 1969; 18(5):1071-81. DOI: 10.1016/0006-2952(69)90111-7. View

12.

Sapolsky R . Cellular defenses against excitotoxic insults. J Neurochem. 2001; 76(6):1601-11. DOI: 10.1046/j.1471-4159.2001.00203.x. View

13.

Jellinger K . Basic mechanisms of neurodegeneration: a critical update. J Cell Mol Med. 2010; 14(3):457-87. PMC: 3823450. DOI: 10.1111/j.1582-4934.2010.01010.x. View

14.

Barrette B, Hebert M, Filali M, Lafortune K, Vallieres N, Gowing G . Requirement of myeloid cells for axon regeneration. J Neurosci. 2008; 28(38):9363-76. PMC: 6671109. DOI: 10.1523/JNEUROSCI.1447-08.2008. View

15.

Levin S, BUCCI T, Cohen S, Fix A, Hardisty J, LeGrand E . The nomenclature of cell death: recommendations of an ad hoc Committee of the Society of Toxicologic Pathologists. Toxicol Pathol. 1999; 27(4):484-90. DOI: 10.1177/019262339902700419. View

16.

Kvachnina E, Liu G, Dityatev A, Renner U, Dumuis A, Richter D . 5-HT7 receptor is coupled to G alpha subunits of heterotrimeric G12-protein to regulate gene transcription and neuronal morphology. J Neurosci. 2005; 25(34):7821-30. PMC: 6725246. DOI: 10.1523/JNEUROSCI.1790-05.2005. View

17.

Murrain M, Murphy A, Mills L, Kater S . Neuron-specific modulation by serotonin of regenerative outgrowth and intracellular calcium within the CNS of Helisoma trivolvis. J Neurobiol. 1990; 21(4):611-8. DOI: 10.1002/neu.480210408. View

18.

Kalil K, Szebenyi G, Dent E . Common mechanisms underlying growth cone guidance and axon branching. J Neurobiol. 2000; 44(2):145-58. View

19.

Schaefer A, Schoonderwoert V, Ji L, Mederios N, Danuser G, Forscher P . Coordination of actin filament and microtubule dynamics during neurite outgrowth. Dev Cell. 2008; 15(1):146-62. PMC: 2595147. DOI: 10.1016/j.devcel.2008.05.003. View

20.

Nicholls D . Mitochondrial dysfunction and glutamate excitotoxicity studied in primary neuronal cultures. Curr Mol Med. 2004; 4(2):149-77. DOI: 10.2174/1566524043479239. View