Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2018 Aug 22

PMID 30128251

Citations 13

Authors

Simge G Yuz

Julia Ricken

Seraphine V Wegner

Affiliations

Soon will be listed here.

Abstract

Independent control over multiple cell-material interactions with high spatiotemporal resolution is a key for many biomedical applications and understanding cell biology, as different cell types can perform different tasks in a multicellular context. In this study, the binding of two different cell types to materials is orthogonally controlled with blue and red light providing independent regulation in space and time. Cells expressing the photoswitchable protein cryptochrome 2 (CRY2) on cell surface bind to N-truncated CRY-interacting basic helix-loop-helix protein 1 (CIBN)-immobilized substrates under blue light and cells expressing the photoswitchable protein phytochrome B (PhyB ) on cell surface bind to phytochrome interaction factor 6 (PIF6)-immobilized substrates under red light, respectively. These light-switchable cell interactions provide orthogonal and noninvasive control using two wavelengths of visible light. Moreover, both cell-material interactions are dynamically switched on under light and reversible in the dark. The specificity of the CRY2/CIBN and PhyB/PIF6 interactions and their response to different wavelengths of light allow selectively activating the binding of one cell type with blue and the other cell type with red light in the presence of the other cell type.

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References

Muller K, Engesser R, Metzger S, Schulz S, Kampf M, Busacker M . A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. Nucleic Acids Res. 2013; 41(7):e77. PMC: 3627562. DOI: 10.1093/nar/gkt002. View

Valon L, Marin-Llaurado A, Wyatt T, Charras G, Trepat X . Optogenetic control of cellular forces and mechanotransduction. Nat Commun. 2017; 8:14396. PMC: 5309899. DOI: 10.1038/ncomms14396. View

Custodio C, Frias A, Del Campo A, Reis R, Mano J . Selective cell recruitment and spatially controlled cell attachment on instructive chitosan surfaces functionalized with antibodies. Biointerphases. 2012; 7(1-4):65. DOI: 10.1007/s13758-012-0065-3. View

Liu D, Xie Y, Shao H, Jiang X . Using azobenzene-embedded self-assembled monolayers to photochemically control cell adhesion reversibly. Angew Chem Int Ed Engl. 2009; 48(24):4406-8. DOI: 10.1002/anie.200901130. View

Chandra R, Douglas E, Mathies R, Bertozzi C, Francis M . Programmable cell adhesion encoded by DNA hybridization. Angew Chem Int Ed Engl. 2005; 45(6):896-901. DOI: 10.1002/anie.200502421. View

Muller K, Weber W . Optogenetic tools for mammalian systems. Mol Biosyst. 2013; 9(4):596-608. DOI: 10.1039/c3mb25590e. View

Gomez E, Gerhardt K, Judd J, Tabor J, Suh J . Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. ACS Nano. 2015; 10(1):225-37. DOI: 10.1021/acsnano.5b05558. View

Li W, Wang J, Ren J, Qu X . Near-infrared upconversion controls photocaged cell adhesion. J Am Chem Soc. 2014; 136(6):2248-51. DOI: 10.1021/ja412364m. View

Duan L, Che D, Zhang K, Ong Q, Guo S, Cui B . Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol. 2015; 22(5):671-82. PMC: 4443846. DOI: 10.1016/j.chembiol.2015.04.014. View

10.

Kennedy M, Hughes R, Peteya L, Schwartz J, Ehlers M, Tucker C . Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 2010; 7(12):973-5. PMC: 3059133. DOI: 10.1038/nmeth.1524. View

11.

Yuz S, Ricken J, Wegner S . Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci (Weinh). 2018; 5(8):1800446. PMC: 6097145. DOI: 10.1002/advs.201800446. View

12.

Polstein L, Gersbach C . A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015; 11(3):198-200. PMC: 4412021. DOI: 10.1038/nchembio.1753. View

13.

Yu G, Onodera H, Aono Y, Kawano F, Ueda Y, Furuya A . Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. Sci Rep. 2016; 6:35777. PMC: 5073340. DOI: 10.1038/srep35777. View

14.

Auernheimer J, Dahmen C, Hersel U, Bausch A, Kessler H . Photoswitched cell adhesion on surfaces with RGD peptides. J Am Chem Soc. 2005; 127(46):16107-10. DOI: 10.1021/ja053648q. View

15.

Robertus J, Browne W, Feringa B . Dynamic control over cell adhesive properties using molecular-based surface engineering strategies. Chem Soc Rev. 2009; 39(1):354-78. DOI: 10.1039/b906608j. View

16.

Levskaya A, Weiner O, Lim W, Voigt C . Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009; 461(7266):997-1001. PMC: 2989900. DOI: 10.1038/nature08446. View

17.

Douglas E, Chandra R, Bertozzi C, Mathies R, Francis M . Self-assembled cellular microarrays patterned using DNA barcodes. Lab Chip. 2007; 7(11):1442-8. DOI: 10.1039/b708666k. View

18.

Elbert D . Bottom-up tissue engineering. Curr Opin Biotechnol. 2011; 22(5):674-80. PMC: 3153565. DOI: 10.1016/j.copbio.2011.04.001. View

19.

Lee T, Garcia J, Paez J, Singh A, Phelps E, Weis S . Light-triggered in vivo activation of adhesive peptides regulates cell adhesion, inflammation and vascularization of biomaterials. Nat Mater. 2014; 14(3):352-60. PMC: 4336636. DOI: 10.1038/nmat4157. View

20.

Rechenmacher F, Neubauer S, Polleux J, Mas-Moruno C, De Simone M, Cavalcanti-Adam E . Functionalizing αvβ3- or α5β1-selective integrin antagonists for surface coating: a method to discriminate integrin subtypes in vitro. Angew Chem Int Ed Engl. 2013; 52(5):1572-5. DOI: 10.1002/anie.201206370. View