Virulence Gene Profiles and Molecular Genetic Characteristics of Diarrheagenic from a Hospital in Western China

Overview

Journal Gut Pathog

Publisher Biomed Central

Specialty Gastroenterology

Date 2018 Aug 22

PMID 30127859

Citations 8

Authors

Dan Li

Min Shen

Ying Xu

Chao Liu

Wen Wang

Jinyan Wu

Xianmei Luo

Xu Jia

Yongxin Ma

Affiliations

Soon will be listed here.

Abstract

Background: Diarrheagenic (DEC) is one of the most important etiological agents of diarrheal diseases. In this study we investigated the prevalence, virulence gene profiles, antimicrobial resistance, and molecular genetic characteristics of DEC at a hospital in western China.

Methods: A total of 110 clinical isolates were collected from the First Affiliated Hospital of Chengdu Medical College from 2015 to 2016. Microbiological methods, PCR, antimicrobial susceptibility test, pulsed-field gel electrophoresis and multilocus sequence typing were used in this study.

Results: Molecular analysis of six DEC pathotype marker genes showed that 13 of the 110 isolates (11.82%) were DEC including nine (8.18%) diffusely adherent (DAEC) and four (3.64%) enteroaggregative (EAEC). The adherence genes and were present in all DAEC and EAEC isolates. All nine DAEC isolates harbored the virulence genes and and four (44.44%) also carried the and genes. The virulence genes , , , , and were found in 100%, 100%, 75%, 50%, and 50% of EAEC isolates, respectively. In addition, all DEC isolates were multidrug resistant and had high frequencies of antimicrobial resistance. Molecular genetic characterization showed that the 13 DEC isolates were divided into 11 pulsed-field gel electrophoresis patterns and 10 sequence types.

Conclusions: To the best of our knowledge, this study provides the first report of DEC, including DAEC and EAEC, in western China. Our analyses identified the virulence genes present in from a hospital indicating their role in the isolated DEC strains' pathogenesis. At the same time, the analyses revealed, the antimicrobial resistance pattern of the DEC isolates. Thus, DAEC and EAEC among the DEC strains should be considered a significant risk to humans in western China due to their evolved pathogenicity and antimicrobial resistance pattern.

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References

Chakraborty S, Deokule J, Garg P, Bhattacharya S, Nandy R, Nair G . Concomitant infection of enterotoxigenic Escherichia coli in an outbreak of cholera caused by Vibrio cholerae O1 and O139 in Ahmedabad, India. J Clin Microbiol. 2001; 39(9):3241-6. PMC: 88325. DOI: 10.1128/JCM.39.9.3241-3246.2001. View

Watanabe H, Arakawa E, Ito K, Kato J, Nakamura A . Genetic analysis of an invasion region by use of a Tn3-lac transposon and identification of a second positive regulator gene, invE, for cell invasion of Shigella sonnei: significant homology of invE with ParB of plasmid P1. J Bacteriol. 1990; 172(2):619-29. PMC: 208485. DOI: 10.1128/jb.172.2.619-629.1990. View

Lau S, Reddy S, Cheesbrough J, Bolton F, Willshaw G, Cheasty T . Major uropathogenic Escherichia coli strain isolated in the northwest of England identified by multilocus sequence typing. J Clin Microbiol. 2008; 46(3):1076-80. PMC: 2268376. DOI: 10.1128/JCM.02065-07. View

Kaper J, Nataro J, Mobley H . Pathogenic Escherichia coli. Nat Rev Microbiol. 2004; 2(2):123-40. DOI: 10.1038/nrmicro818. View

Wong A, Pearson J, Bright M, Munera D, Robinson K, Lee S . Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements. Mol Microbiol. 2011; 80(6):1420-38. DOI: 10.1111/j.1365-2958.2011.07661.x. View

Qu M, Lv B, Zhang X, Yan H, Huang Y, Qian H . Prevalence and antibiotic resistance of bacterial pathogens isolated from childhood diarrhea in Beijing, China (2010-2014). Gut Pathog. 2016; 8:31. PMC: 4906916. DOI: 10.1186/s13099-016-0116-2. View

Barletta F, Ochoa T, Cleary T . Multiplex real-time PCR (MRT-PCR) for diarrheagenic. Methods Mol Biol. 2012; 943:307-14. DOI: 10.1007/978-1-60327-353-4_21. View

Rakin A, Schneider L, Podladchikova O . Hunger for iron: the alternative siderophore iron scavenging systems in highly virulent Yersinia. Front Cell Infect Microbiol. 2012; 2:151. PMC: 3510459. DOI: 10.3389/fcimb.2012.00151. View

Jonsson R, Struve C, Boisen N, Mateiu R, Santiago A, Jenssen H . Novel aggregative adherence fimbria variant of enteroaggregative Escherichia coli. Infect Immun. 2015; 83(4):1396-405. PMC: 4363450. DOI: 10.1128/IAI.02820-14. View

10.

Vossenkamper A, Macdonald T, Marches O . Always one step ahead: How pathogenic bacteria use the type III secretion system to manipulate the intestinal mucosal immune system. J Inflamm (Lond). 2011; 8:11. PMC: 3094202. DOI: 10.1186/1476-9255-8-11. View

11.

Elliott S, Sperandio V, Giron J, Shin S, Mellies J, Wainwright L . The locus of enterocyte effacement (LEE)-encoded regulator controls expression of both LEE- and non-LEE-encoded virulence factors in enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun. 2000; 68(11):6115-26. PMC: 97688. DOI: 10.1128/IAI.68.11.6115-6126.2000. View

12.

Elias Jr W, Czeczulin J, Henderson I, Trabulsi L, Nataro J . Organization of biogenesis genes for aggregative adherence fimbria II defines a virulence gene cluster in enteroaggregative Escherichia coli. J Bacteriol. 1999; 181(6):1779-85. PMC: 93575. DOI: 10.1128/JB.181.6.1779-1785.1999. View

13.

McDaniel T, Jarvis K, Donnenberg M, Kaper J . A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci U S A. 1995; 92(5):1664-8. PMC: 42580. DOI: 10.1073/pnas.92.5.1664. View

14.

Schubert S, Rakin A, Karch H, Carniel E, Heesemann J . Prevalence of the "high-pathogenicity island" of Yersinia species among Escherichia coli strains that are pathogenic to humans. Infect Immun. 1998; 66(2):480-5. PMC: 107931. DOI: 10.1128/IAI.66.2.480-485.1998. View

15.

Muller D, Greune L, Heusipp G, Karch H, Fruth A, Tschape H . Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR. Appl Environ Microbiol. 2007; 73(10):3380-90. PMC: 1907121. DOI: 10.1128/AEM.02855-06. View

16.

Linhartova I, Bumba L, Masin J, Basler M, Osicka R, Kamanova J . RTX proteins: a highly diverse family secreted by a common mechanism. FEMS Microbiol Rev. 2010; 34(6):1076-112. PMC: 3034196. DOI: 10.1111/j.1574-6976.2010.00231.x. View

17.

Nataro J, Kaper J . Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1):142-201. PMC: 121379. DOI: 10.1128/CMR.11.1.142. View

18.

Preethirani P, Isloor S, Sundareshan S, Nuthanalakshmi V, Deepthikiran K, Sinha A . Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India. PLoS One. 2015; 10(11):e0142717. PMC: 4654528. DOI: 10.1371/journal.pone.0142717. View

19.

De Rycke J, Gonzalez E, Blanco J, Oswald E, Blanco M, Boivin R . Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. J Clin Microbiol. 1990; 28(4):694-9. PMC: 267779. DOI: 10.1128/jcm.28.4.694-699.1990. View

20.

Gartner J, Schmidt M . Comparative analysis of locus of enterocyte effacement pathogenicity islands of atypical enteropathogenic Escherichia coli. Infect Immun. 2004; 72(11):6722-8. PMC: 523029. DOI: 10.1128/IAI.72.11.6722-6728.2004. View