The Active Immunoglobulin Kappa Chain Gene is Packaged by Non-ubiquitin-conjugated Nucleosomes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Jun 1

PMID 3012532

Citations 11

Authors

S Y Huang

M B Barnard

M Xu

S Matsui

S M Rose

W T Garrard

Affiliations

Soon will be listed here.

Abstract

To elucidate the molecular features of active chromatin, we have mapped, by two-dimensional electrophoresis, the protein composition of nucleosomes that package the immunoglobulin kappa chain gene of mouse plasmacytoma cells. Nucleoprotein particles that possess the active kappa chain gene comigrate with bulk mononucleosomes that contain high mobility group proteins HMG-14 or -17 but lack histone H1. High electrophoretic resolution of the underlying core particles, after removal of ubiquitin by isopeptidase treatment, reveals that these nucleosomes are nonubiquitinated, even though they coincidently migrate with bulk ubiquitinated particles. This distinctive electrophoretic behavior may be correlated with the presence of histone H2A.X. Nucleosomes exhibiting these unusual properties appear to span at least 10 kilobases, in both transcribed and nontranscribed regions, suggesting that mechanisms independent of transcription exist to initiate, maintain, and propagate a common chromatin phenotype over long distances along the kappa chain locus.

Citing Articles

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References

Thomas J, Kornberg R . An octamer of histones in chromatin and free in solution. Proc Natl Acad Sci U S A. 1975; 72(7):2626-30. PMC: 432822. DOI: 10.1073/pnas.72.7.2626. View

Reudelhuber T, Ball D, Davis A, Garrard W . Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA. Nucleic Acids Res. 1982; 10(4):1311-25. PMC: 320527. DOI: 10.1093/nar/10.4.1311. View

Beyer A, Christensen M, Walker B, Lestourgeon W . Identification and characterization of the packaging proteins of core 40S hnRNP particles. Cell. 1977; 11(1):127-38. DOI: 10.1016/0092-8674(77)90323-3. View

McKnight S, Bustin M, MILLER Jr O . Electron microscopic analysis of chromosome metabolism in the Drosophila melanogaster embryo. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 2:741-54. DOI: 10.1101/sqb.1978.042.01.075. View

Zweidler A . Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents. Methods Cell Biol. 1978; 17:223-33. View

Schibler U, Marcu K, PERRY R . The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells. Cell. 1978; 15(4):1495-509. DOI: 10.1016/0092-8674(78)90072-7. View

Albright S, Nelson P, Garrard W . Histone molar ratios among different electrophoretic forms of mono- and dinucleosomes. J Biol Chem. 1979; 254(4):1065-73. View

Albright S, WISEMAN J, Lange R, Garrard W . Subunit structures of different electrophoretic forms of nucleosomes. J Biol Chem. 1980; 255(8):3673-84. View

Laskey R . The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods Enzymol. 1980; 65(1):363-71. DOI: 10.1016/s0076-6879(80)65047-2. View

10.

Matsui S, Sandberg A, Negoro S, Seon B, Goldstein G . Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds. Proc Natl Acad Sci U S A. 1982; 79(5):1535-9. PMC: 346009. DOI: 10.1073/pnas.79.5.1535. View

11.

Richards R, Shaw B . In situ protamine release: a versatile sample preparation method for the electrophoretic analysis of nuclear proteins on acid/urea-based gels. Anal Biochem. 1982; 121(1):69-82. DOI: 10.1016/0003-2697(82)90558-9. View

12.

Baer B, Rhodes D . Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes. Nature. 1983; 301(5900):482-8. DOI: 10.1038/301482a0. View

13.

Hershko A . Ubiquitin: roles in protein modification and breakdown. Cell. 1983; 34(1):11-2. DOI: 10.1016/0092-8674(83)90131-9. View

14.

Ball D, Gross D, Garrard W . 5-methylcytosine is localized in nucleosomes that contain histone H1. Proc Natl Acad Sci U S A. 1983; 80(18):5490-4. PMC: 384283. DOI: 10.1073/pnas.80.18.5490. View

15.

Einck L, Bustin M . Inhibition of transcription in somatic cells by microinjection of antibodies to chromosomal proteins. Proc Natl Acad Sci U S A. 1983; 80(22):6735-9. PMC: 390060. DOI: 10.1073/pnas.80.22.6735. View

16.

BELLARD M, Dretzen G, Bellard F, Oudet P, Chambon P . Disruption of the typical chromatin structure in a 2500 base-pair region at the 5' end of the actively transcribed ovalbumin gene. EMBO J. 1982; 1(2):223-30. PMC: 553024. DOI: 10.1002/j.1460-2075.1982.tb01151.x. View

17.

Finley D, Ciechanover A, Varshavsky A . Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85. Cell. 1984; 37(1):43-55. DOI: 10.1016/0092-8674(84)90299-x. View

18.

Ciechanover A, Finley D, Varshavsky A . Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85. Cell. 1984; 37(1):57-66. DOI: 10.1016/0092-8674(84)90300-3. View

19.

Rose S, Garrard W . Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes. J Biol Chem. 1984; 259(13):8534-44. View

20.

Kanda F, Matsui S, Sykes D, Sandberg A . Affinity of chromatin constituents for isopeptidase. Biochem Biophys Res Commun. 1984; 122(3):1296-306. DOI: 10.1016/0006-291x(84)91233-6. View