Transgenic Sheep Generated Via OPS Vitrified-microinjected Pronuclear Embryos and Reproduction Efficiency of the Transgenic Offspring

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2018 Aug 21

PMID 30123717

Citations 6

Authors

Xiuzhi Tian

Dongying Lv

Teng Ma

Shoulong Deng

Minghui Yang

Yukun Song

Xiaosheng Zhang

Jinglong Zhang

Juncai Fu

Zhengxing Lian

Shien Zhu

Yingjie Wu

Yiming Xing

Guoshi Liu

Affiliations

Soon will be listed here.

Abstract

Background: The open pulled straw (OPS) vitrification method has been successfully applied in mouse, pig, and goat embryos as well as in buffalo oocytes, but it has not yet been applied to the microinjected embryos. This study examined the effects of OPS vitrification on embryo development and the reproductive capacity of the transgenic offspring in order to establish a method for preservation of microinjected embryos.

Methods: Ovine pronuclear embryos were microinjected with the exogenous aralkylamine N-acetyltransferase gene (), frozen by the OPS method, and subsequently thawed for embryo transplantation. Pregnancy rate, lambing rate, survival rate, average birth weight and transgenic positive rate as well as reproduction efficiency and hormone level of the transgenic offspring were investigated to analyze the effect of OPS vitrification on microinjectd pronuclear embryos.

Results: No significant differences were observed in the birth rate, lamb survival rate and transgenic positive rate between the frozen and non-frozen -microinjected pronuclear embryos. The average birth weight of the frozen embryos offspring was greater than that of the non-frozen embryos. Importantly, the transgenic offspring that overexpressed the gene showed improved ovulation efficiency and lambing rate by regulating their hormone levels.

Conclusions: The OPS vitrification approach may be a valuable method in microinjected- embryo transfer technology, which could reserve embryos and result in fewer unnecessary animal sacrifices. In addition, the + transgenic offspring exhibited improved reproductive capacity on account of regulation effect of melatonin on reproductive hormone. These data may provide available references for human-assisted reproduction.

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References

Hammer R, Pursel V, Rexroad Jr C, Wall R, Bolt D, Ebert K . Production of transgenic rabbits, sheep and pigs by microinjection. Nature. 1985; 315(6021):680-3. DOI: 10.1038/315680a0. View

Vajta G, Holm P, Greve T, Callesen H . Vitrification of porcine embryos using the Open Pulled Straw (OPS) method. Acta Vet Scand. 1997; 38(4):349-52. PMC: 8057035. View

Willadsen S, Polge C, ROWSON L, Moor R . Deep freezing of sheep embryos. J Reprod Fertil. 1976; 46(1):151-4. DOI: 10.1530/jrf.0.0460151. View

Hardeland R, Madrid J, Tan D, Reiter R . Melatonin, the circadian multioscillator system and health: the need for detailed analyses of peripheral melatonin signaling. J Pineal Res. 2011; 52(2):139-66. DOI: 10.1111/j.1600-079X.2011.00934.x. View

Smith A, Simon P, Moller O, Scholler R, McNeilly A . Effect of melatonin implantation on the seasonal variation of FSH secretion in the male blue fox (Alopex lagopus). J Reprod Fertil. 1988; 83(1):345-54. DOI: 10.1530/jrf.0.0830345. View

Nishijima K, Liu E, Yamaguchi S, Tanaka M, Morimoto M, Watanabe T . Delaying embryo development by storing at 4°C for synchronization to recipients in microinjection technique in rabbits. Lab Anim. 2012; 47(1):53-7. DOI: 10.1258/la.2012.012097. View

Kasiraj R, Misra A, Mutha Rao M, Jaiswal R, Rangareddi N . Successful culmination of pregnancy and live birth following the transfer of frozen-thawed buffalo embryos. Theriogenology. 1993; 39(5):1187-92. DOI: 10.1016/0093-691x(93)90016-x. View

Uchida M, Shimatsu Y, Onoe K, Matsuyama N, Niki R, Ikeda J . Production of transgenic miniature pigs by pronuclear microinjection. Transgenic Res. 2002; 10(6):577-82. DOI: 10.1023/a:1013059917280. View

Wong J, Wong A . Phasing-in of vitrification into routine practice: why, how, and what. Hong Kong Med J. 2011; 17(2):119-26. View

10.

Cseh S, Corselli J, Nehlsen-Cannarella S, Bailey L, Szalay A . The effect of quick-freezing in ethylene glycol on morphological survival and in vitro development of mouse embryos frozen at different preimplantation stages. Theriogenology. 1997; 48(1):43-50. DOI: 10.1016/S0093-691X(97)00190-8. View

11.

WHITTINGHAM D, Leibo S, Mazur P . Survival of mouse embryos frozen to -196 degrees and -269 degrees C. Science. 1972; 178(4059):411-4. View

12.

Poulton A, English J, Symons A, Arendt J . Changes in plasma concentrations of LH, FSH and prolactin in ewes receiving melatonin and short-photoperiod treatments to induce early onset of breeding activity. J Endocrinol. 1987; 112(1):103-11. DOI: 10.1677/joe.0.1120103. View

13.

Soliman A, Lacasse A, Lanoix D, Sagrillo-Fagundes L, Boulard V, Vaillancourt C . Placental melatonin system is present throughout pregnancy and regulates villous trophoblast differentiation. J Pineal Res. 2015; 59(1):38-46. DOI: 10.1111/jpi.12236. View

14.

Reiter R, Mayo J, Tan D, Sainz R, Alatorre-Jimenez M, Qin L . Melatonin as an antioxidant: under promises but over delivers. J Pineal Res. 2016; 61(3):253-78. DOI: 10.1111/jpi.12360. View

15.

Su J, Wang Y, Xing X, Zhang L, Sun H, Zhang Y . Melatonin significantly improves the developmental competence of bovine somatic cell nuclear transfer embryos. J Pineal Res. 2015; 59(4):455-68. DOI: 10.1111/jpi.12275. View

16.

Vergouw C, Kostelijk E, Doejaaren E, Hompes P, Lambalk C, Schats R . The influence of the type of embryo culture medium on neonatal birthweight after single embryo transfer in IVF. Hum Reprod. 2012; 27(9):2619-26. DOI: 10.1093/humrep/des252. View

17.

Dhali A, Manik R, Das S, Singla S, Palta P . Vitrification of buffalo (Bubalus bubalis) oocytes. Theriogenology. 2000; 53(6):1295-303. DOI: 10.1016/s0093-691x(00)00273-9. View

18.

Tian X, Wang F, Zhang L, Ji P, Wang J, Lv D . Melatonin Promotes the In Vitro Development of Microinjected Pronuclear Mouse Embryos via Its Anti-Oxidative and Anti-Apoptotic Effects. Int J Mol Sci. 2017; 18(5). PMC: 5454901. DOI: 10.3390/ijms18050988. View

19.

Park S, Lee D, Jang H, Byeon Y, Kim Y, Back K . Melatonin-rich transgenic rice plants exhibit resistance to herbicide-induced oxidative stress. J Pineal Res. 2012; 54(3):258-63. DOI: 10.1111/j.1600-079X.2012.01029.x. View

20.

Wang B, Baldassarre H, Tao T, Gauthier M, Neveu N, Zhou J . Transgenic goats produced by DNA pronuclear microinjection of in vitro derived zygotes. Mol Reprod Dev. 2002; 63(4):437-43. DOI: 10.1002/mrd.10199. View