A Conserved Cis-acting Sequence in the 5' Leader of Avian Sarcoma Virus RNA is Required for Packaging

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1986 Jul 1

PMID 3012114

Citations 82

Authors

R A KATZ

R W Terry

A M Skalka

Affiliations

Soon will be listed here.

Abstract

The deletion of a conserved sequence of ca. 30 nucleotides in the 5' noncoding leader region of an avian sarcoma virus DNA clone resulted in a loss of infectivity after transfection of chicken embryo fibroblasts. Genetic and biochemical analysis of a representative mutant demonstrated that the env gene was expressed normally. Thus, viral RNA transcription, splicing, and translation were not impaired. The amount of mutant viral RNA encapsidated into virions, however, was severely reduced despite the presence of helper-virus. We conclude that the deleted sequence is an essential cis-acting packaging signal.

Citing Articles

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Rous Sarcoma Virus Genomic RNA Dimerization Capability In Vitro Is Not a Prerequisite for Viral Infectivity.

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Identification of conserved, primary sequence motifs that direct retrovirus RNA fate.

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References

Duff R, Vogt P . Characteristics of two new avian tumor virus subgroups. Virology. 1969; 39(1):18-30. DOI: 10.1016/0042-6822(69)90344-4. View

KATZ R, Cullen B, Malavarca R, Skalka A . Role of the avian retrovirus mRNA leader in expression: evidence for novel translational control. Mol Cell Biol. 1986; 6(2):372-9. PMC: 367526. DOI: 10.1128/mcb.6.2.372-379.1986. View

Stacey D, Allfrey V, Hanafusa H . Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs. Proc Natl Acad Sci U S A. 1977; 74(4):1614-8. PMC: 430841. DOI: 10.1073/pnas.74.4.1614. View

Legerski R, HODNETT J, Gray Jr H . Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes. Nucleic Acids Res. 1978; 5(5):1445-64. PMC: 342095. DOI: 10.1093/nar/5.5.1445. View

Stacey D . Messenger activity of virion RNA for avian leukosis viral envelope glycoprotein. J Virol. 1979; 29(3):949-56. PMC: 353254. DOI: 10.1128/JVI.29.3.949-956.1979. View

Shank P, Linial M . Avian oncovirus mutant (SE21Q1b) deficient in genomic RNA: characterization of a deletion in the provirus. J Virol. 1980; 36(2):450-6. PMC: 353661. DOI: 10.1128/JVI.36.2.450-456.1980. View

Goff S, Traktman P, Baltimore D . Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. J Virol. 1981; 38(1):239-48. PMC: 171145. DOI: 10.1128/JVI.38.1.239-248.1981. View

Hackett P, Swanstrom R, Varmus H, Bishop J . The leader sequence of the subgenomic mRNA's of Rous sarcoma virus is approximately 390 nucleotides. J Virol. 1982; 41(2):527-34. PMC: 256781. DOI: 10.1128/JVI.41.2.527-534.1982. View

Schwartz D, Tizard R, Gilbert W . Nucleotide sequence of Rous sarcoma virus. Cell. 1983; 32(3):853-69. DOI: 10.1016/0092-8674(83)90071-5. View

10.

Scholl D, Kahn S, Malavarca R, Astrin S, Skalka A . Nucleotide sequence of the long terminal repeat and flanking cellular sequences of avian endogenous retrovirus ev-2: variation in Rous-associated virus-0 expression cannot be explained by differences in primary sequence. J Virol. 1983; 45(2):868-71. PMC: 256482. DOI: 10.1128/JVI.45.2.868-871.1983. View

11.

Schwartzberg P, Colicelli J, Goff S . Deletion mutants of Moloney murine leukemia virus which lack glycosylated gag protein are replication competent. J Virol. 1983; 46(2):538-46. PMC: 255156. DOI: 10.1128/JVI.46.2.538-546.1983. View

12.

Watanabe S, Temin H . Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene. Proc Natl Acad Sci U S A. 1982; 79(19):5986-90. PMC: 347036. DOI: 10.1073/pnas.79.19.5986. View

13.

Pugatsch T, Stacey D . Identification of a sequence likely to be required for avian retroviral packaging. Virology. 1983; 128(2):505-11. DOI: 10.1016/0042-6822(83)90279-9. View

14.

Sorge J, Ricci W, Hughes S . cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus. J Virol. 1983; 48(3):667-75. PMC: 255399. DOI: 10.1128/JVI.48.3.667-675.1983. View

15.

Lerner T, Hanafusa H . DNA sequence of the Bryan high-titer strain of Rous sarcoma virus: extent of env deletion and possible genealogical relationship with other viral strains. J Virol. 1984; 49(2):549-56. PMC: 255496. DOI: 10.1128/JVI.49.2.549-556.1984. View

16.

Bizub D, KATZ R, Skalka A . Nucleotide sequence of noncoding regions in Rous-associated virus-2: comparisons delineate conserved regions important in replication and oncogenesis. J Virol. 1984; 49(2):557-65. PMC: 255497. DOI: 10.1128/JVI.49.2.557-565.1984. View

17.

Cullen B, Lomedico P, Ju G . Transcriptional interference in avian retroviruses--implications for the promoter insertion model of leukaemogenesis. Nature. 1984; 307(5948):241-5. DOI: 10.1038/307241a0. View

18.

Ficht T, Chang L, Stoltzfus C . Avian sarcoma virus gag and env gene structural protein precursors contain a common amino-terminal sequence. Proc Natl Acad Sci U S A. 1984; 81(2):362-6. PMC: 344676. DOI: 10.1073/pnas.81.2.362. View

19.

Kopchick J, Stacey D . Differences in intracellular DNA ligation after microinjection and transfection. Mol Cell Biol. 1984; 4(2):240-6. PMC: 368687. DOI: 10.1128/mcb.4.2.240-246.1984. View

20.

Koyama T, Harada F, Kawai S . Characterization of a Rous sarcoma virus mutant defective in packaging its own genomic RNA: biochemical properties of mutant TK15 and mutant-induced transformants. J Virol. 1984; 51(1):154-62. PMC: 254413. DOI: 10.1128/JVI.51.1.154-162.1984. View