Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2018 Aug 18

PMID 30116486

Citations 18

Authors

Xu Zheng

Bin Jia

Xiao-Ting Tian

Xue Song

Mo-Li Wu

Qing-You Kong

Hong Li

Jia Liu

Affiliations

Soon will be listed here.

Abstract

Anaplastic thyroid carcinoma (ATC) is the most lethal thyroid malignancy without a reliable therapeutic agent. Resveratrol possesses cancer-suppressive effects, while its effect(s) on ATC cells remains unknown. Because oxidative damage caused by increased reactive oxygen species (ROS) is one of the therapeutic effects of anticancer drugs and oxidative stress-caused mitochondria swelling is observed in resveratrol-treated cancer cells, the oxidative statuses and their relevance with resveratrol sensitivities are elucidated using THJ-16T and THJ-11T ATC cells established from two human anaplastic thyroid carcinoma cases. The results revealed that resveratrol-treated THJ-16T rather than THJ-11T cells showed remarkable growth arrest and extensive apoptosis accompanied with the elevated ROS generation and the attenuated superoxide dismutase 2 (SOD2) and catalase (CAT) levels. Mitochondrial impairment and the enhanced caspase-9/caspase-3 activation are found only in resveratrol-sensitive THJ-16T cells. Treatment with the antioxidant N-acetylcysteine (NAC) partly attenuated resveratrol-induced ROS generation and apoptosis of THJ-16T cells. The levels of resveratrol metabolic enzymes (SULT1A1 and SULT1C2) in THJ-16T cells were lower than those in THJ-11T cells and therefore reversely related with resveratrol sensitivities of ATC cells. Our findings demonstrate the ability of resveratrol to increase ROS generation and oxidative-related cellular lesions in resveratrol-sensitive THJ-16T cells presumably through activating the ROS-mitochondrial signal pathway. The levels of SULTs and ROS may reflect the response manners of ATC cells to resveratrol.

Citing Articles

State of Knowledge About Thyroid Cancers in the Era of COVID-19-A Narrative Review.

Bronowicka-Szydelko A, Rabczynski M, Dumas I, Fiodorenko-Dumas Z, Wojtczak B, Kotyra L Biomedicines. 2025; 12(12.

PMID: 39767735 PMC: 11672969. DOI: 10.3390/biomedicines12122829.

The Growth Inhibitory Effect of Resveratrol and Gallic Acid on Prostate Cancer Cell Lines through the Alteration of Oxidative Stress Balance: The Interplay between , and Genes.

Moghadam D, Zarei R, Rostami A, Samare-Najaf M, Ghojoghi R, Savardashtaki A Anticancer Agents Med Chem. 2024; 24(16):1220-1232.

PMID: 38984567 DOI: 10.2174/0118715206317999240708062744.

Research progress of SIRTs activator resveratrol and its derivatives in autoimmune diseases.

Yu X, Chen M, Wu J, Song R Front Immunol. 2024; 15:1390907.

PMID: 38962006 PMC: 11219927. DOI: 10.3389/fimmu.2024.1390907.

Promising Approaches in Plant-Based Therapies for Thyroid Cancer: An Overview of In Vitro, In Vivo, and Clinical Trial Studies.

Kaczmarzyk I, Nowak-Perlak M, Wozniak M Int J Mol Sci. 2024; 25(8).

PMID: 38674046 PMC: 11050626. DOI: 10.3390/ijms25084463.

Research progress of plant-derived natural products in thyroid carcinoma.

Du Q, Shen W Front Chem. 2024; 11:1279384.

PMID: 38268761 PMC: 10806030. DOI: 10.3389/fchem.2023.1279384.

References

Maryam A, Mehmood T, Zhang H, Li Y, Khan M, Ma T . Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress. Sci Rep. 2017; 7(1):6242. PMC: 5524969. DOI: 10.1038/s41598-017-06535-y. View

Hematpoor A, Paydar M, Liew S, Sivasothy Y, Mohebali N, Looi C . Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways. Chem Biol Interact. 2017; 279:210-218. DOI: 10.1016/j.cbi.2017.11.014. View

Li Y, Tian X, Wu M, Zheng X, Kong Q, Cheng X . Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells. Int J Mol Sci. 2018; 19(4). PMC: 5979404. DOI: 10.3390/ijms19041030. View

Chedea V, Vicas S, Sticozzi C, Pessina F, Frosini M, Maioli E . Resveratrol: from diet to topical usage. Food Funct. 2017; 8(11):3879-3892. DOI: 10.1039/c7fo01086a. View

Hanschmann E, Godoy J, Berndt C, Hudemann C, Lillig C . Thioredoxins, glutaredoxins, and peroxiredoxins--molecular mechanisms and health significance: from cofactors to antioxidants to redox signaling. Antioxid Redox Signal. 2013; 19(13):1539-605. PMC: 3797455. DOI: 10.1089/ars.2012.4599. View

Chapa-Dubocq X, Makarov V, Javadov S . Simple kinetic model of mitochondrial swelling in cardiac cells. J Cell Physiol. 2017; 233(7):5310-5321. PMC: 6063534. DOI: 10.1002/jcp.26335. View

Kovacic P, Somanathan R . Multifaceted approach to resveratrol bioactivity: Focus on antioxidant action, cell signaling and safety. Oxid Med Cell Longev. 2010; 3(2):86-100. PMC: 2952093. DOI: 10.4161/oxim.3.2.11147. View

Wilke J, Kawamura T, Watanabe N, Osada H, Ziegler S, Waldmann H . Identification of cytotoxic, glutathione-reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion. Bioorg Med Chem. 2017; 26(8):1453-1461. DOI: 10.1016/j.bmc.2017.11.009. View

Jacobsen A, Groholt K, Lorntzsen B, Osnes T, Falk R, Sigstad E . Anaplastic thyroid cancer and hyperfractionated accelerated radiotherapy (HART) with and without surgery. Eur Arch Otorhinolaryngol. 2017; 274(12):4203-4209. DOI: 10.1007/s00405-017-4764-8. View

10.

Dewangan J, Tandon D, Srivastava S, Verma A, Yapuri A, Rath S . Novel combination of salinomycin and resveratrol synergistically enhances the anti-proliferative and pro-apoptotic effects on human breast cancer cells. Apoptosis. 2017; 22(10):1246-1259. DOI: 10.1007/s10495-017-1394-y. View

11.

Wu M, Li H, Yu L, Chen X, Kong Q, Song X . Short-term resveratrol exposure causes in vitro and in vivo growth inhibition and apoptosis of bladder cancer cells. PLoS One. 2014; 9(2):e89806. PMC: 3934942. DOI: 10.1371/journal.pone.0089806. View

12.

Xue S, Xiao-Hong S, Lin S, Jie B, Li-Li W, Jia-Yao G . Lumbar puncture-administered resveratrol inhibits STAT3 activation, enhancing autophagy and apoptosis in orthotopic rat glioblastomas. Oncotarget. 2016; 7(46):75790-75799. PMC: 5342778. DOI: 10.18632/oncotarget.12414. View

13.

Ko Y, Chang C, Chien H, Wu C, Lin L . Resveratrol enhances the expression of death receptor Fas/CD95 and induces differentiation and apoptosis in anaplastic large-cell lymphoma cells. Cancer Lett. 2011; 309(1):46-53. DOI: 10.1016/j.canlet.2011.05.014. View

14.

Chen X, Liu R, Liu X, Xu C, Wang X . L-ascorbic Acid-2-Glucoside inhibits Helicobacter pylori-induced apoptosis through mitochondrial pathway in Gastric Epithelial cells. Biomed Pharmacother. 2017; 97:75-81. DOI: 10.1016/j.biopha.2017.10.030. View

15.

Lin S, Lin J, Hsueh C, Chou T, Wong R . Effects of roniciclib in preclinical models of anaplastic thyroid cancer. Oncotarget. 2017; 8(40):67990-68000. PMC: 5620230. DOI: 10.18632/oncotarget.19092. View

16.

Sies H, Berndt C, Jones D . Oxidative Stress. Annu Rev Biochem. 2017; 86:715-748. DOI: 10.1146/annurev-biochem-061516-045037. View

17.

Molinaro E, Romei C, Biagini A, Sabini E, Agate L, Mazzeo S . Anaplastic thyroid carcinoma: from clinicopathology to genetics and advanced therapies. Nat Rev Endocrinol. 2017; 13(11):644-660. DOI: 10.1038/nrendo.2017.76. View

18.

Shu X, Li H, Sun Z, Wu M, Ma J, Wang J . Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells. Biochem Pharmacol. 2010; 79(10):1516-25. DOI: 10.1016/j.bcp.2010.01.022. View

19.

Galadari S, Rahman A, Pallichankandy S, Thayyullathil F . Reactive oxygen species and cancer paradox: To promote or to suppress?. Free Radic Biol Med. 2017; 104:144-164. DOI: 10.1016/j.freeradbiomed.2017.01.004. View

20.

Ranganath R, Shah M, Shah A . Anaplastic thyroid cancer. Curr Opin Endocrinol Diabetes Obes. 2015; 22(5):387-91. DOI: 10.1097/MED.0000000000000189. View