The Methionine Initiator TRNA Genes of Yeast

Overview

Journal Gene

Specialty Molecular Biology

Date 1986 Jan 1

PMID 3011608

Citations 9

Authors

A M Cigan

T F Donahue

Affiliations

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Abstract

We have isolated three distinct tRNAimet genes from a yeast DNA clone bank. The complete sequence of two shows that these genes are colinear with the mature tRNAimet and supports the RNA sequence of tRNAimet. Southern analysis of yeast genomic DNA indicates the presence of four copies of tRNAimet gene per haploid genome.

Citing Articles

GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in the maturation of initiator methionyl-tRNA in Saccharomyces cerevisiae.

Calvo O, Cuesta R, Anderson J, Gutierrez N, Hinnebusch A, Tamame M Mol Cell Biol. 1999; 19(6):4167-81.

PMID: 10330157 PMC: 104376. DOI: 10.1128/MCB.19.6.4167.

The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA.

Anderson J, Phan L, Cuesta R, Carlson B, Pak M, Asano K Genes Dev. 1998; 12(23):3650-62.

PMID: 9851972 PMC: 317256. DOI: 10.1101/gad.12.23.3650.

A very poorly expressed tRNA(Ser) is highly concentrated together with replication primer initiator tRNA(Met) in the yeast Ty1 virus-like particles.

Pochart P, Agoutin B, Fix C, Keith G, HEYMAN T Nucleic Acids Res. 1993; 21(7):1517-21.

PMID: 8386834 PMC: 309356. DOI: 10.1093/nar/21.7.1517.

Modulation of tRNA(iMet), eIF-2, and eIF-2B expression shows that GCN4 translation is inversely coupled to the level of eIF-2.GTP.Met-tRNA(iMet) ternary complexes.

Dever T, Yang W, Astrom S, Bystrom A, Hinnebusch A Mol Cell Biol. 1995; 15(11):6351-63.

PMID: 7565788 PMC: 230887. DOI: 10.1128/MCB.15.11.6351.

Sigma elements are position-specific for many different yeast tRNA genes.

Sandmeyer S, Bilanchone V, Clark D, Morcos P, Carle G, Brodeur G Nucleic Acids Res. 1988; 16(4):1499-515.

PMID: 3279393 PMC: 336330. DOI: 10.1093/nar/16.4.1499.