The Effect of Plasma Pretreatment and Cross-Linking Degree on the Physical and Antimicrobial Properties of Nisin-Coated PVA Films

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2018 Aug 18

PMID 30115861

Citations 6

Authors

Zuzana Kolarova Raskova

Pavel Stahel

Jana Sedlarikova

Lenka Musilova

Monika Stupavska

Marian Lehocky

Affiliations

Soon will be listed here.

Abstract

Stable antimicrobial nisin layers were prepared on the carrying medium-polyvinyl alcohol (PVA) films, crosslinked by glutaric acid. Surface plasma dielectric coplanar surface barrier discharge (DCSBD) modification of polyvinyl alcohol was used to improve the hydrophilic properties and to provide better adhesion of biologically active peptide-nisin to the polymer. The surface modification of films was studied in correlation to their cross-linking degree. Nisin was attached directly from the salt solution of the commercial product. In order to achieve a stable layer, the initial nisin concentration and the following release were investigated using chromatographic methods. The uniformity and stability of the layers was evaluated by means of zeta potential measurements, and for the surface changes of hydrophilic character, the water contact angle measurements were provided. The nisin long-term stability on the PVA films was confirmed by tricine polyacrylamide gel electrophoresis (SDS-PAGE) and by antimicrobial assay. It was found that PVA can serve as a suitable carrying medium for nisin with tunable properties by plasma treatment and crosslinking degree.

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References

Sze A, Erickson D, Ren L, Li D . Zeta-potential measurement using the Smoluchowski equation and the slope of the current-time relationship in electroosmotic flow. J Colloid Interface Sci. 2005; 261(2):402-10. DOI: 10.1016/S0021-9797(03)00142-5. View

Duan J, Park S, Daeschel M, Zhao Y . Antimicrobial chitosan-lysozyme (CL) films and coatings for enhancing microbial safety of mozzarella cheese. J Food Sci. 2007; 72(9):M355-62. DOI: 10.1111/j.1750-3841.2007.00556.x. View

Cho D, Lee S, Frey M . Characterizing zeta potential of functional nanofibers in a microfluidic device. J Colloid Interface Sci. 2012; 372(1):252-60. DOI: 10.1016/j.jcis.2012.01.007. View

Schilke K, McGuire J . Detection of nisin and fibrinogen adsorption on poly(ethylene oxide) coated polyurethane surfaces by time-of-flight secondary ion mass spectrometry (TOF-SIMS). J Colloid Interface Sci. 2011; 358(1):14-24. PMC: 3074594. DOI: 10.1016/j.jcis.2011.03.014. View

Dorgan K, Wooderchak W, Wynn D, Karschner E, Alfaro J, Cui Y . An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases. Anal Biochem. 2006; 350(2):249-55. DOI: 10.1016/j.ab.2006.01.004. View

Ryder M, Schilke K, Auxier J, McGuire J, Neff J . Nisin adsorption to polyethylene oxide layers and its resistance to elution in the presence of fibrinogen. J Colloid Interface Sci. 2010; 350(1):194-9. PMC: 2918692. DOI: 10.1016/j.jcis.2010.06.038. View

Zasada K, Lukasiewicz-Atanasov M, Klysik K, Lewandowska-Lancucka J, Gzyl-Malcher B, Puciul-Malinowska A . 'One-component' ultrathin multilayer films based on poly(vinyl alcohol) as stabilizing coating for phenytoin-loaded liposomes. Colloids Surf B Biointerfaces. 2015; 135:133-142. DOI: 10.1016/j.colsurfb.2015.07.033. View

Gubskaya A, Khan I, Valenzuela L, Lisnyak Y, Kohn J . Investigating the Release of a Hydrophobic Peptide from Matrices of Biodegradable Polymers: An Integrated Method Approach. Polymer (Guildf). 2013; 54(15):3806-3820. PMC: 3770487. DOI: 10.1016/j.polymer.2013.05.038. View

Bilek F, Krizova T, Lehocky M . Preparation of active antibacterial LDPE surface through multistep physicochemical approach: I. Allylamine grafting, attachment of antibacterial agent and antibacterial activity assessment. Colloids Surf B Biointerfaces. 2011; 88(1):440-7. DOI: 10.1016/j.colsurfb.2011.07.027. View

10.

Wisniewski J, Gaugaz F . Fast and sensitive total protein and Peptide assays for proteomic analysis. Anal Chem. 2015; 87(8):4110-6. DOI: 10.1021/ac504689z. View

11.

Adamczyk Z, Nattich M, Wasilewska M, Zaucha M . Colloid particle and protein deposition - electrokinetic studies. Adv Colloid Interface Sci. 2011; 168(1-2):3-28. DOI: 10.1016/j.cis.2011.04.002. View

12.

Fang D, Chen Y, Xu B, Ren K, He Z, He L . Development of lipid-shell and polymer core nanoparticles with water-soluble salidroside for anti-cancer therapy. Int J Mol Sci. 2014; 15(3):3373-88. PMC: 3975343. DOI: 10.3390/ijms15033373. View

13.

Olle Resa C, Jagus R, Gerschenson L . Effect of natamycin, nisin and glycerol on the physicochemical properties, roughness and hydrophobicity of tapioca starch edible films. Mater Sci Eng C Mater Biol Appl. 2014; 40:281-7. DOI: 10.1016/j.msec.2014.04.005. View