Superoxide Anion Generating Capacity and Lysosomal Enzyme Activities of Kupffer Cells in Galactosamine Induced Hepatitis

Overview

Journal Gastroenterol Jpn

Specialty Gastroenterology

Date 1986 Apr 1

PMID 3011577

Citations 5

Authors

Y Shiratori

H Takikawa

T Kawase

T Sugimoto

Affiliations

Soon will be listed here.

Abstract

To elucidate the function of the reticuloendothelial system of liver in hepatic injury, we investigated the effect of endotoxins on superoxide anion (O-2) generating capacity and lysosomal enzyme activities of Kupffer cells isolated from rats treated with galactosamine (Gal N), with Gal N supplemented with polymyxin B (Polymyxin B-Gal N), with lipopolysaccharide (LPS) and from control rats. After collagenase digestion of the liver and centrifugation over metrizamide gradient, Kupffer cells were prepared by the dish adherence procedure. O-2 production by the cells was examined as chemiluminescence during phagocytosis of latex particles and beta-glucuronidase activities were analyzed. High titers of endotoxemia were detected in LPS and Gal N rats by limulus test, while a low endotoxemia titer was found in Polymyxin B-Gal N rats. Hepatocyte damage was found in Gal N rats, but little was recognized in LPS and Polymyxin B-Gal N rats. In the latter groups, Kupffer cells, activated by endotoxins, showed the enhancement of chemiluminescence and a release of lysosomal enzyme. Though lysosomal enzyme was released from Kupffer cells in Gal N rats, chemiluminescence was slightly suppressed in spite of the high titer of endotoxemia. These results appear to be related to the consumption of O-2 during liver injury. The functional state of Kupffer cells was thus changed by the grade of endotoxemia and hepatic injury.

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References

JOHNSTON Jr R, Godzik C, COHN Z . Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J Exp Med. 1978; 148(1):115-27. PMC: 2184904. DOI: 10.1084/jem.148.1.115. View

Weiss S, LoBuglio A, Kessler H . Oxidative mechanisms of monocyte-mediated cytotoxicity. Proc Natl Acad Sci U S A. 1980; 77(1):584-7. PMC: 348318. DOI: 10.1073/pnas.77.1.584. View

Berg T, Seljelid R . Distribution of lysosomal enzymes in different types of rat liver cells. Exp Cell Res. 1976; 99(1):146-54. DOI: 10.1016/0014-4827(76)90689-3. View

Lopes J, Inniss W . Electron microscopy of effect of polymyxin on Escherichia coli lipopolysaccharide. J Bacteriol. 1969; 100(2):1128-9. PMC: 250208. DOI: 10.1128/jb.100.2.1128-1130.1969. View

Tanner A, Keyhani A, Reiner R, Holdstock G, Wright R . Proteolytic enzymes released by liver macrophages may promote hepatic injury in a rat model of hepatic damage. Gastroenterology. 1981; 80(4):647-54. View

Alexander P, Evans R . Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971; 232(29):76-8. DOI: 10.1038/newbio232076a0. View

Broitman S, GOTTLIEB L, ZAMCHECK N . INFLUENCE OF NEOMYCIN AND INGESTED ENDOTOXIN IN THE PATHOGENESIS OF CHOLINE DEFICIENCY CIRRHOSIS IN THE ADULT RAT. J Exp Med. 1964; 119:633-42. PMC: 2137847. DOI: 10.1084/jem.119.4.633. View

Weiss S, Slivka A . Monocyte and granulocyte-mediated tumor cell destruction. A role for the hydrogen peroxide-myeloperoxidase-chloride system. J Clin Invest. 1982; 69(2):255-62. PMC: 370973. DOI: 10.1172/jci110447. View

Hibbs Jr J . Heterocytolysis by macrophages activated by bacillus Calmette-Guérin: lysosome exocytosis into tumor cells. Science. 1974; 184(4135):468-71. DOI: 10.1126/science.184.4135.468. View

10.

Weening R, Wever R, Roos D . Quantitative aspects of the production of superoxide radicals by phagocytizing human granulocytes. J Lab Clin Med. 1975; 85(2):245-52. View

11.

GIANETTO R, DE DUVE C . Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles. Biochem J. 1955; 59(3):433-8. PMC: 1216262. DOI: 10.1042/bj0590433. View

12.

Rubin R, Farber J . Mechanisms of the killing of cultured hepatocytes by hydrogen peroxide. Arch Biochem Biophys. 1984; 228(2):450-9. DOI: 10.1016/0003-9861(84)90010-9. View

13.

Berry M, Friend D . High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969; 43(3):506-20. PMC: 2107801. DOI: 10.1083/jcb.43.3.506. View

14.

Weissmann G, Thomas L . Studies on lysosomes. I. The effects of endotoxin, endotoxin tolerance, and cortisone on the release of acid hydrolases from a granular fraction of rabbit liver. J Exp Med. 1962; 116:433-50. PMC: 2137624. DOI: 10.1084/jem.116.4.433. View

15.

Shiratori Y, KAMII K, Murao S . Kupffer cells inhibit the lymphoproliferative response to antigenic stimulation by rat hepatocytes. Scand J Gastroenterol. 1983; 18(3):411-5. DOI: 10.3109/00365528309181616. View

16.

Pabst M, JOHNSTON Jr R . Increased production of superoxide anion by macrophages exposed in vitro to muramyl dipeptide or lipopolysaccharide. J Exp Med. 1980; 151(1):101-14. PMC: 2185752. DOI: 10.1084/jem.151.1.101. View

17.

Drath D, Karnovsky M . Superoxide production by phagocytic leukocytes. J Exp Med. 1975; 141(1):257-62. PMC: 2190499. DOI: 10.1084/jem.141.1.257. View

18.

FERLUGA J, Allison A . Role of mononuclear infiltrating cells in pathogenesis of hepatitis. Lancet. 1978; 2(8090):610-1. DOI: 10.1016/s0140-6736(78)92828-3. View

19.

Hirata K, Kaneko A, Ogawa K, Hayasaka H, Onoe T . Effect of endotoxin on rat liver. Analysis of acid phosphatase isozymes in the liver of normal and endotoxin-treated rats. Lab Invest. 1980; 43(2):165-71. View

20.

Nolan J . Endotoxin, reticuloendothelial function, and liver injury. Hepatology. 1981; 1(5):458-65. DOI: 10.1002/hep.1840010516. View