Heteroantisera Prepared Against B Lymphocytes at Different Stages of Differentiation. I. Preparation of Sera and Cytotoxicity to Lymphoid Cells from Different Organs

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1977 May 1

PMID 301115

Citations 2

Authors

R M Gorczynski

Affiliations

Soon will be listed here.

Abstract

A routine method is described whereby anti-mouse B-cell antisera can be raised in rabbits. As judged by trypan blue dye exclusion these sera have negligible cytotoxicity to murine T lymphocytes but seem to detect differences in non-T lymphocytes in different organs. By sedimentation analysis some of the sera may detect (in addition to mature B cells) earlier precursors of mature B lymphocytes in bone marrow and spleen.

Citing Articles

Differentiation of functionally active mouse T-lymphocytes from functionally inactive bone marrow precursors.

Gorczynski R, MacRae S Immunology. 1977; 33(5):697-712.

PMID: 145406 PMC: 1445508.

Effect of gold sodium thiomalate on murine lymphocyte functions.

JENNINGS J, MacRae S, Gorczynski R Clin Exp Immunol. 1979; 36(2):260-5.

PMID: 113153 PMC: 1537737.

References

Schlesinger M . Antigens of the thymus. Prog Allergy. 1972; 16:214-99. DOI: 10.1159/000313173. View

Takahashi T, Old L, MCINTIRE K, Boyse E . Immunoglobulin and other surface antigens of cells of the immune system. J Exp Med. 1971; 134(4):815-32. PMC: 2139003. DOI: 10.1084/jem.134.4.815. View

OSMOND D, Nossal G . Differentiation of lymphocytes in mouse bone marrow. II. Kinetics of maturation and renewal of antiglobulin-binding cells studied by double labeling. Cell Immunol. 1974; 13(1):132-45. DOI: 10.1016/0008-8749(74)90233-0. View

Shortman K . Separation methods for lymphocyte populations. Contemp Top Mol Immunol. 1974; 3:161-203. DOI: 10.1007/978-1-4684-2838-4_7. View

Trizio D, CUDKOWICZ G . Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo. J Immunol. 1974; 113(4):1093-7. View

Julius M, Simpson E, Herzenberg L . A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973; 3(10):645-9. DOI: 10.1002/eji.1830031011. View

Strober S . Immune function cell surface characteristics and maturation of B cell subpopulations. Transplant Rev. 1975; 24:84-112. DOI: 10.1111/j.1600-065x.1975.tb00166.x. View

Miller R, Gorczynski R, Lafleur L, MacDonald H, Phillips R . Cell separation analysis of B and T lymphocyte differentiation. Transplant Rev. 1975; 25:59-97. DOI: 10.1111/j.1600-065x.1975.tb00726.x. View

Shortman K, Jackson H . The differentiation of T lymphocytes. I. Proliferation kinetics and interrelationships of subpopulations of mouse thymus cells. Cell Immunol. 1974; 12(2):230-46. DOI: 10.1016/0008-8749(74)90075-6. View

10.

Lafleur L, Miller R, Phillips R . A quantitative assay for the progenitors of bone marrow-associated lymphocytes. J Exp Med. 1972; 135(6):1363-74. PMC: 2139178. DOI: 10.1084/jem.135.6.1363. View

11.

Gorczynski R, Miller R, Phillips R . Identification by density separation of antigen-specific surface receptors on the progenitors of antibody-producing cells. Immunology. 1971; 20(5):693-705. PMC: 1455865. View

12.

CANTOR H, Boyse E . Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975; 141(6):1376-89. PMC: 2189856. DOI: 10.1084/jem.141.6.1376. View

13.

Parish C, Chilcott A . Functional significance of the complement receptors on B lymphocytes. Cell Immunol. 1975; 20(2):290-303. DOI: 10.1016/0008-8749(75)90106-9. View

14.

Gronowicz E, Coutinho A . Heterogeneity of B cells: direct evidence of selective triggering of distinct subpopulations by polyclonal activators. Scand J Immunol. 1976; 5(1-2):55-69. DOI: 10.1111/j.1365-3083.1976.tb02992.x. View

15.

Gorczynski R, Feldmann M . B Cell heterogeneity - difference in the size of B lymphocytes responding to T dependent and T independent antigens. Cell Immunol. 1975; 18(1):88-97. DOI: 10.1016/0008-8749(75)90039-8. View

16.

Raff M . Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969; 224(5217):378-9. DOI: 10.1038/224378a0. View

17.

Miller R, Phillips R . Separation of cells by velocity sedimentation. J Cell Physiol. 1969; 73(3):191-201. DOI: 10.1002/jcp.1040730305. View