Insertion of Transposon Tn7 into the Escherichia Coli GlmS Transcriptional Terminator

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Feb 15

PMID 3010949

Citations 23

Authors

N J Gay

V L Tybulewicz

J E Walker

Affiliations

Soon will be listed here.

Abstract

The transposon Tn7 is unusual as it transposes at high frequencies from episomal elements to a unique site in the Escherichia coli chromosome. This unique site is within a region of dyad symmetry that we have demonstrated to be the transcriptional terminator of the glmS gene which encodes the glutamine amidotransferase, glucosamine synthetase. Transposition of Tn7 abolishes termination of glmS transcription at this site; the transcripts now extend into the left end of Tn7 and terminate at a new site, tm, 127 base pairs from the left end of Tn7. This region of the transposon contains a long open reading frame which encodes a protein sequence that is significantly related to the transposase proteins of the transposable elements IS1 and Tn3. A weak transcript has been identified that emanates from a promoter on the 5' side of this reading frame. This promoter is over-run by glmS transcripts and so it appears that expression of the Tn7 transposase may be regulated by promoter occlusion.

Citing Articles

Annotation and Comparative Genomics of Prokaryotic Transposable Elements.

Ross K, Zerillo M, Chandler M, Varani A Methods Mol Biol. 2024; 2802:189-213.

PMID: 38819561 DOI: 10.1007/978-1-0716-3838-5_8.

Novel molecular requirements for CRISPR RNA-guided transposition.

Walker M, Klompe S, Zhang D, Sternberg S Nucleic Acids Res. 2023; 51(9):4519-4535.

PMID: 37078593 PMC: 10201428. DOI: 10.1093/nar/gkad270.

A Surface Exposed, Two-Domain Lipoprotein Cargo of a Type XI Secretion System Promotes Colonization of Host Intestinal Epithelia Expressing Glycans.

Grossman A, Escobar C, Mans E, Mucci N, Mauer T, Jones K Front Microbiol. 2022; 13:800366.

PMID: 35572647 PMC: 9100927. DOI: 10.3389/fmicb.2022.800366.

FlgM as a secretion moiety for the development of an inducible type III secretion system.

Heel T, Vogel G, Lammirato A, Schneider R, Auer B PLoS One. 2013; 8(3):e59034.

PMID: 23554966 PMC: 3595227. DOI: 10.1371/journal.pone.0059034.

Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7.

Mitra R, McKenzie G, Yi L, Lee C, Craig N Mob DNA. 2010; 1(1):18.

PMID: 20653944 PMC: 2918618. DOI: 10.1186/1759-8753-1-18.

References

McLachlan A . Tests for comparing related amino-acid sequences. Cytochrome c and cytochrome c 551 . J Mol Biol. 1971; 61(2):409-24. DOI: 10.1016/0022-2836(71)90390-1. View

Walker J, Gay N, Saraste M, Eberle A . DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS. Biochem J. 1984; 224(3):799-815. PMC: 1144516. DOI: 10.1042/bj2240799. View

Barth P, Datta N, Hedges R, Grinter N . Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. J Bacteriol. 1976; 125(3):800-10. PMC: 236152. DOI: 10.1128/jb.125.3.800-810.1976. View

Imada A, Nozaki Y, Kawashima F, Yoneda M . Regulation of glucosamine utilization in Staphylococcus aureus and Escherichia coli. J Gen Microbiol. 1977; 100(2):329-37. DOI: 10.1099/00221287-100-2-329. View

Barth P, Grinter N, BRADLEY D . Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion. J Bacteriol. 1978; 133(1):43-52. PMC: 221974. DOI: 10.1128/jb.133.1.43-52.1978. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

SANGER F, Coulson A . The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978; 87(1):107-10. DOI: 10.1016/0014-5793(78)80145-8. View

Ohtsubo H, Ohtsubo E . Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A. 1978; 75(2):615-9. PMC: 411306. DOI: 10.1073/pnas.75.2.615. View

Berk A, Sharp P . Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978; 75(3):1274-8. PMC: 411453. DOI: 10.1073/pnas.75.3.1274. View

10.

Adhya S, Gottesman M . Control of transcription termination. Annu Rev Biochem. 1978; 47:967-96. DOI: 10.1146/annurev.bi.47.070178.004535. View

11.

Fennewald M, Shapiro J . Transposition of Tn7 in Pseudomonas aeruginosa and isolation of alk::Tn7 mutations. J Bacteriol. 1979; 139(1):264-9. PMC: 216854. DOI: 10.1128/jb.139.1.264-269.1979. View

12.

Heffron F, McCarthy B, Ohtsubo H, Ohtsubo E . DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell. 1979; 18(4):1153-63. DOI: 10.1016/0092-8674(79)90228-9. View

13.

Karn J, Brenner S, Barnett L, Cesareni G . Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci U S A. 1980; 77(9):5172-6. PMC: 350019. DOI: 10.1073/pnas.77.9.5172. View

14.

Lichtenstein C, Brenner S . Unique insertion site of Tn7 in the E. coli chromosome. Nature. 1982; 297(5867):601-3. DOI: 10.1038/297601a0. View

15.

Ely B . Transposition of Tn7 occurs at a single site on the Caulobacter crescentus chromosome. J Bacteriol. 1982; 151(2):1056-8. PMC: 220364. DOI: 10.1128/jb.151.2.1056-1058.1982. View

16.

Staden R . An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Res. 1982; 10(9):2951-61. PMC: 320667. DOI: 10.1093/nar/10.9.2951. View

17.

Adhya S, Gottesman M . Promoter occlusion: transcription through a promoter may inhibit its activity. Cell. 1982; 29(3):939-44. DOI: 10.1016/0092-8674(82)90456-1. View

18.

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

19.

Farrell P, Deininger P, Bankier A, Barrell B . Homologous upstream sequences near Epstein-Barr virus promoters. Proc Natl Acad Sci U S A. 1983; 80(6):1565-9. PMC: 393642. DOI: 10.1073/pnas.80.6.1565. View

20.

Biggin M, Gibson T, Hong G . Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983; 80(13):3963-5. PMC: 394179. DOI: 10.1073/pnas.80.13.3963. View