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Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage Via PI3K/Akt/eNOS Pathway

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Date 2018 Aug 16
PMID 30108656
Citations 7
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Abstract

Danggui Sini decoction (DSD) is a traditional Chinese decoction, which is wildly applied and showed to be effective in ameliorating ischemia-related symptoms. However, the mechanisms of DSD action in ischemic damage remain to be fully clarified. Pancreatic islet endothelial cells are pivotal constituent of islet microvasculature, with high vulnerability to hypoxic injuries. Here, using MST1 cell, a pancreatic islet endothelial cell-line, as a model, we investigated the effects of DSD on hypoxia-stimulated endothelial cell lesions and its underlying mechanisms. We found that DSD-Containing Serum (DSD-CS), collected from DSD-treated rats, could efficiently protect MST1 survival and proliferation from Cobalt chloride (CoCl) induced damage, including cell viability, proliferation, and tube formation. Furthermore, DSD-CS restored the activity of PI3K/Akt/eNOS signaling inhibited by CoCl in MST1 cells. The protective effect of DSD-CS could be blocked by the specific PI3K/Akt/eNOS inhibitor LY294002, suggesting that DSD-CS protection of MST1 cell survival from hypoxia was mediated by PI3K/Akt/eNOS pathway. In conclusion, DSD treatment protected MST1 survival from hypoxic injuries via PI3K/Akt/eNOS pathway, indicating its role in protecting microvascular endothelial cells.

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References
1.
Johansson M, Mattsson G, Andersson A, Jansson L, Carlsson P . Islet endothelial cells and pancreatic beta-cell proliferation: studies in vitro and during pregnancy in adult rats. Endocrinology. 2006; 147(5):2315-24. DOI: 10.1210/en.2005-0997. View

2.
Guo J, Chakraborty A, Liu P, Gan W, Zheng X, Inuzuka H . pVHL suppresses kinase activity of Akt in a proline-hydroxylation-dependent manner. Science. 2016; 353(6302):929-32. PMC: 5326551. DOI: 10.1126/science.aad5755. View

3.
Zheng F, Wei P, Huo H, Xing X, Chen F, Tan X . Neuroprotective effect of gui zhi (ramulus cinnamomi) on ma huang- (herb ephedra-) induced toxicity in rats treated with a ma huang-gui zhi herb pair. Evid Based Complement Alternat Med. 2015; 2015:913461. PMC: 4321680. DOI: 10.1155/2015/913461. View

4.
Schnitzer J . Update on the cellular and molecular basis of capillary permeability. Trends Cardiovasc Med. 2011; 3(4):124-30. DOI: 10.1016/1050-1738(93)90012-U. View

5.
Zheng H, Qin X, Song H, Tang C, Ruan J, Zhang H . [¹H-NMR based metabonomic approach to evaluate anti-coagulant effect of Danggui Sini decoction]. Zhongguo Zhong Yao Za Zhi. 2016; 40(20):4088-93. View