Immunization with Chlamydia Psittaci Plasmid-encoded Protein CPSIT_p7 Induces Partial Protective Immunity Against Chlamydia Lung Infection in Mice

Overview

Journal Immunol Res

Specialty Allergy & Immunology

Date 2018 Aug 12

PMID 30097797

Citations 6

Authors

Yuan Tan

Yumeng Li

Yang Zhang

Jian Yu

Yating Wen

Chuan Wang

Man Xu

Qian Chen

Chunxue Lu

Yimou Wu

Affiliations

Soon will be listed here.

Abstract

The present study evaluated the immune-protective efficacy of the Chlamydia psittaci (C. psittaci) plasmid protein CPSIT_p7 and analyzed the potential mechanisms of this protection. The current study used recombinant CPSIT_p7 protein with Freund's complete adjuvant and Freund's incomplete adjuvant to vaccinate BALB/c mice. Adjuvants alone or PBS formulated with the same adjuvants was used as negative controls. Mice were intranasally challenged with 10 inclusion-forming units (IFU) of C. psittaci. We found that CPSIT_p7 vaccination significantly decreased the mouse lung chlamydial load, interferon-γ (IFN-γ) level, and pathological injury. This protection correlated well with specific humoral and cellular immune responses against C. psittaci. In vitro or in vivo neutralization of C. psittaci with sera harvested from immunized mice did not reduce the number of recoverable C. psittaci in the infected lungs, but CD4 spleen cells collected from CPSIT_p7-immunized mice significantly decreased the chlamydial load via adoptive transfer to native mice. These results reveal that the protection conferred by CPSIT_p7 is dependent on CD4 T cells.

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