All Three IP Receptor Subtypes Generate Ca Puffs, the Universal Building Blocks of IP-evoked Ca Signals

Overview

Journal J Cell Sci

Specialty Cell Biology

Date 2018 Aug 12

PMID 30097556

Citations 22

Authors

Stefania Mataragka

Colin W Taylor

Affiliations

Soon will be listed here.

Abstract

All three subtypes of inositol 1,4,5-trisphosphate receptor (IPR) are intracellular Ca channels that are co-regulated by IP and Ca This allows IPRs to evoke regenerative Ca signals, the smallest of which are Ca puffs that reflect the coordinated opening of a few clustered IPRs. We use total internal reflection microscopy (TIRF) microscopy to record Ca signals in HEK cells expressing all three IPR subtypes or a single native subtype. Ca puffs are less frequent in cells expressing one IPR subtype, commensurate with them expressing fewer IPRs than wild-type cells. However, all three IPR subtypes generate broadly similar Ca puffs with similar numbers of IPRs contributing to each. This suggests that IPR clusters may be assembled by conserved mechanisms that generate similarly sized clusters across different IPR expression levels. The Ca puffs evoked by IPR2 had slower kinetics and more prolonged durations, which may be due to IP binding with greater affinity to IPR2. We conclude that Ca puffs are the building blocks for the Ca signals evoked by all IPRs.

Citing Articles

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