Activities of Deglycosylated Thyrotropin at the Thyroid Membrane Receptor-adenylate Cyclase System

Overview

Journal J Endocrinol Invest

Publisher Springer

Specialty Endocrinology

Date 1985 Dec 1

PMID 3009595

Citations 2

Authors

S Amr

Y Shimohigashi

H C Chen

B Nisula

B D Weintraub

Affiliations

Soon will be listed here.

Abstract

A bovine thyrotropin (bTSH) preparation was deglycosylated by treatment with anhydrous hydrogen fluoride (HF) in the presence of anisole. The resulting material consisted of TSH derivatives that exhibited different molecular sizes, all smaller than the native hormone. The majority (62%) of the deglycosylated TSH derivatives did not bind to the lectin concanavalin A, while 98% of the native TSH was able to bind. The deglycosylated TSH derivatives bound to the high affinity-high specificity TSH binding sites in human thyroid membranes with a potency more than twice that of equivalent immunological amounts of the native bTSH. Despite the enhanced binding affinity for the TSH receptor, the deglycosylated TSH derivatives were unable to stimulate adenylate cyclase fully. Maximal stimulation achieved with bTSH derivatives was only 9 to 17% of the maximal stimulation achieved with native bTSH. Further, the deglycosylated derivatives competitively inhibited stimulation of the thyroidal adenylate cyclase by native bTSH. We conclude that HF treatment of bTSH results in partially deglycosylated TSH derivatives that exhibit enhanced ability to bind to the TSH receptor and markedly diminished adenylate cyclase-stimulating activity.

Citing Articles

Intrinsic bioactivity of thyrotropin in human serum is inversely correlated with thyroid hormone concentrations. Application of a new bioassay using the FRTL-5 rat thyroid cell strain.

Dahlberg P, Petrick P, Nissim M, Weintraub B J Clin Invest. 1987; 79(5):1388-94.

PMID: 3571493 PMC: 424398. DOI: 10.1172/JCI112966.

Changes in the sialylation and sulfation of secreted thyrotropin in congenital hypothyroidism.

Gyves P, Gesundheit N, Thotakura N, Stannard B, DeCherney G, Weintraub B Proc Natl Acad Sci U S A. 1990; 87(10):3792-6.

PMID: 1692623 PMC: 53989. DOI: 10.1073/pnas.87.10.3792.

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