HBV Epidemiology and Genetic Diversity in an Area of High Prevalence of Hepatitis B in Southern Brazil

Overview

Journal Braz J Infect Dis

Specialty Infectious Diseases

Date 2018 Aug 10

PMID 30092176

Citations 7

Authors

Juliana De Paoli

Andre Castagna Wortmann

Mirelli Gabardo Klein

Vagner Reinaldo Zingalli Bueno Pereira

Adriana Maria Cirolini

Bibiane Armiliato de Godoy

Nelson Jurandi Rosa Fagundes

Jonas Michel Wolf

Vagner Ricardo Lunge

Daniel Simon

Affiliations

Soon will be listed here.

Abstract

Background: Hepatitis B virus (HBV) infection is a major public health problem in Brazil. HBV endemicity is usually moderate to low according to geographic regions, and high prevalence of this virus has been reported in people of some specific Brazilian counties, including those with a strong influence of Italian colonization in southern Brazil. Analysis of HBV diversity and identification of the main risk factors to HBV infection are necessary to understand hepatitis B epidemiology in these high prevalence regions in southern Brazil.

Objective: To investigate epidemiological characteristics and HBV genotypes and subgenotypes circulating in a specific city with high HBV prevalence.

Methods: A cross-sectional study was performed with 102 HBV chronically infected individuals, recruited in reference outpatient clinics for viral hepatitis in a city of high HBV prevalence (Bento Gonçalves) in Rio Grande do Sul state, Brazil between July and December 2010. Socio-demographic, clinical and behavior-related variables were collected in a structured questionnaire. HBV serological markers (HBsAg, anti-HBc), viral load, genotypes/subgenotypes and drug resistance were evaluated and comparatively analyzed among all patients.

Results: The HBV infected subjects had a mean age of 44.9 (±12.2) years, with 86 patients (84.3%) reporting to have a family history of HBV infection, 51 (50.0%) to share personal objects, and were predominantly of Italian descendants (61; 64.9%). There was a predominance of genotype D (49/54; 90.7%), but genotype A was also detected (5/54; 9.3%). Subgenotypes D1 (1; 4.7%), D2 (3; 14.3%), and D3 (17; 81.0%) were identified. LAM-resistant mutation (rtM204I) and ADV-resistant mutations (rtA181V) were detected in only one patient each.

Conclusions: These results demonstrate a pivotal role of intrafamilial transmission for HBV spreading in this population. Furthermore, there is a high prevalence of HBV genotype D in this region.

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References

Reijnders J, Pas S, Schutten M, de Man R, Janssen H . Entecavir shows limited efficacy in HBeAg-positive hepatitis B patients with a partial virologic response to adefovir therapy. J Hepatol. 2009; 50(4):674-83. DOI: 10.1016/j.jhep.2008.10.033. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Pereira L, Martelli C, Merchan-Hamann E, Montarroyos U, Braga M, de Lima M . Population-based multicentric survey of hepatitis B infection and risk factor differences among three regions in Brazil. Am J Trop Med Hyg. 2009; 81(2):240-7. View

BOOM R, Sol C, Salimans M, Jansen C, van der Noordaa J . Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990; 28(3):495-503. PMC: 269651. DOI: 10.1128/jcm.28.3.495-503.1990. View

Trepo C, Chan H, Lok A . Hepatitis B virus infection. Lancet. 2014; 384(9959):2053-63. DOI: 10.1016/S0140-6736(14)60220-8. View

Barlean L, Saveanu I, Balcos C . Dental patients' attitudes towards infection control. Rev Med Chir Soc Med Nat Iasi. 2014; 118(2):524-7. View

Shepard C, Simard E, Finelli L, Fiore A, Bell B . Hepatitis B virus infection: epidemiology and vaccination. Epidemiol Rev. 2006; 28:112-25. DOI: 10.1093/epirev/mxj009. View

. EASL 2017 Clinical Practice Guidelines on the management of hepatitis B virus infection. J Hepatol. 2017; 67(2):370-398. DOI: 10.1016/j.jhep.2017.03.021. View

Zehender G, Ebranati E, Gabanelli E, Sorrentino C, Lo Presti A, Tanzi E . Enigmatic origin of hepatitis B virus: an ancient travelling companion or a recent encounter?. World J Gastroenterol. 2014; 20(24):7622-34. PMC: 4069291. DOI: 10.3748/wjg.v20.i24.7622. View

10.

Scotto G, Martinelli D, Di Tullio R, Fazio V . Epidemiological and Clinical Features of Hepatitis B Virus Genotypes among Immigrants in Southern Italy. Hepat Res Treat. 2010; 2010:878356. PMC: 3003999. DOI: 10.1155/2010/878356. View

11.

Gusatti C, Costi C, Halon M, Grandi T, Medeiros A, Silva C . Hepatitis B Virus Genotype D Isolates Circulating in Chapecó, Southern Brazil, Originate from Italy. PLoS One. 2015; 10(8):e0135816. PMC: 4537142. DOI: 10.1371/journal.pone.0135816. View

12.

Mojsiejczuk L, Torres C, Sevic I, Badano I, Malan R, Flichman D . Molecular epidemiology of hepatitis B virus in Misiones, Argentina. Infect Genet Evol. 2016; 44:34-42. DOI: 10.1016/j.meegid.2016.06.032. View

13.

Yang S, Wang D, Zhang Y, Yu C, Ren J, Xu K . Transmission of Hepatitis B and C Virus Infection Through Body Piercing: A Systematic Review and Meta-Analysis. Medicine (Baltimore). 2015; 94(47):e1893. PMC: 5058954. DOI: 10.1097/MD.0000000000001893. View

14.

Souto F . Distribution of hepatitis B infection in Brazil: the epidemiological situation at the beginning of the 21 st century. Rev Soc Bras Med Trop. 2015; 49(1):11-23. DOI: 10.1590/0037-8682-0176-2015. View

15.

Ciccozzi M, Chaouch H, Lo Presti A, Taffon S, Villano U, Equestre M . Evolutionary dynamics of HBV-D7 subgenotype in Tunisia. J Med Virol. 2016; 89(3):469-475. DOI: 10.1002/jmv.24665. View

16.

Darriba D, Taboada G, Doallo R, Posada D . jModelTest 2: more models, new heuristics and parallel computing. Nat Methods. 2012; 9(8):772. PMC: 4594756. DOI: 10.1038/nmeth.2109. View

17.

Niederau C . Chronic hepatitis B in 2014: great therapeutic progress, large diagnostic deficit. World J Gastroenterol. 2014; 20(33):11595-617. PMC: 4155353. DOI: 10.3748/wjg.v20.i33.11595. View

18.

Clemente C, Carrilho F, Pinho J, Ono-Nita S, Silva L, Moreira R . A phylogenetic study of hepatitis B virus in chronically infected Brazilian patients of Western and Asian descent. J Gastroenterol. 2009; 44(6):568-76. DOI: 10.1007/s00535-009-0044-8. View

19.

Menegol D, Spilki F . Seroprevalence of Hepatitis B and C markers at the population level in the municipality of Caxias do Sul, southern Brazil. Braz J Microbiol. 2014; 44(4):1237-40. PMC: 3958193. DOI: 10.1590/S1517-83822014005000013. View

20.

Katoh K, Standley D . MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013; 30(4):772-80. PMC: 3603318. DOI: 10.1093/molbev/mst010. View