The Myosin Light-chain Kinase MLCK-1 Relocalizes During Caenorhabditis Elegans Ovulation to Promote Actomyosin Bundle Assembly and Drive Contraction

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2018 Aug 9

PMID 30088798

Citations 10

Authors

Charlotte A Kelley

Alison C E Wirshing

Ronen Zaidel-Bar

Erin J Cram

Affiliations

Soon will be listed here.

Abstract

We identify the Caenorhabditis elegans myosin light-chain kinase, MLCK-1, required for contraction of spermathecae. During contraction, MLCK-1 moves from the apical cell boundaries to the basal actomyosin bundles, where it stabilizes myosin downstream of calcium signaling. MLCK and ROCK act in distinct subsets of cells to coordinate the timing of contraction.

Citing Articles

Tensions on the actin cytoskeleton and apical cell junctions in the spermatheca are influenced by spermathecal anatomy, ovulation state and activation of myosin.

Sadeghian F, Grooms N, Chung S, Cram E Front Cell Dev Biol. 2024; 12:1490803.

PMID: 39474353 PMC: 11518831. DOI: 10.3389/fcell.2024.1490803.

Tensions on the actin cytoskeleton and apical cell junctions in the spermatheca are influenced by spermathecal anatomy, ovulation state and activation of myosin.

Sadeghian F, Grooms N, Chung S, Cram E bioRxiv. 2024; .

PMID: 39282271 PMC: 11398403. DOI: 10.1101/2024.09.03.611016.

Role of ROCK signaling in virus replication.

Kumar R, Barua S, Tripathi B, Kumar N Virus Res. 2023; 329:199105.

PMID: 36977446 PMC: 10194102. DOI: 10.1016/j.virusres.2023.199105.

Tension-dependent RHGF-1 recruitment to stress fibers drives robust spermathecal tissue contraction.

Avivi Kela S, Sethi K, Tan P, Suresh D, Ong H, Castaneda P J Cell Biol. 2022; 222(2).

PMID: 36574264 PMC: 9798103. DOI: 10.1083/jcb.202203105.

FLN-1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub-cellular organelles in a contractile tissue.

Kelley C, Triplett O, Mallick S, Burkewitz K, Mair W, Cram E Cytoskeleton (Hoboken). 2020; 77(10):379-398.

PMID: 32969593 PMC: 7926293. DOI: 10.1002/cm.21633.

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