Monitoring the Action of Redox-directed Cancer Therapeutics Using a Human Peroxiredoxin-2-based Probe

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Aug 9

PMID 30087344

Citations 18

Authors

Troy F Langford

Beijing K Huang

Joseph B Lim

Sun Jin Moon

Hadley D Sikes

Affiliations

Soon will be listed here.

Abstract

Redox cancer therapeutics target the increased reliance on intracellular antioxidant systems and enhanced susceptibility to oxidant-induced stress of some cancer cells compared to normal cells. Many of these therapeutics are thought to perturb intracellular levels of the oxidant hydrogen peroxide (HO), a signaling molecule that modulates a number of different processes in human cells. However, fluorescent probes for this species remain limited in their ability to detect the small perturbations induced during successful treatments. We report a fluorescent sensor based upon human peroxiredoxin-2, which acts as the natural indicator of small HO fluctuations in human cells. The new probe reveals peroxide-induced oxidation in human cells below the detection limit of current probes, as well as peroxiredoxin-2 oxidation caused by two different redox cancer therapeutics in living cells. This capability will be useful in elucidating the mechanism of current redox-based therapeutics and in developing new ones.

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