Histopathological Evaluation of the Exposure by Cyanobacteria Cultive Containing [d-Leu¹]Microcystin-LR on Tadpoles

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2018 Aug 8

PMID 30082615

Citations 1

Authors

Osmindo Rodrigues Pires Junior

Natiela Beatriz de Oliveira

Renan J Bosque

Maria Fernanda Nice Ferreira

Veronica Morais Aurelio da Silva

Ana Carolina Martins Magalhaes

Carlos Jose Correia de Santana

Mariana de Souza Castro

Affiliations

Soon will be listed here.

Abstract

This study evaluated the effects of [d-Leu¹]Microcystin-LR variant by the exposure of tadpole to unialgal culture NPLJ-4 strain. The Tadpole was placed in aquariums and exposed to culture or disrupted cells. For 16 days, 5 individuals were removed every 2 days, and tissue samples of liver, skeletal muscle, and intestinal tract were collected for histopathology and bioaccumulation analyses. After exposure, those surviving tadpoles were placed in clean water for 15 days to evaluate their recovery. A control without algae and toxins was maintained in the same conditions and exhibited normal histology and no tissue damage. In exposed tadpoles, samples were characterized by serious damages that similarly affected the different organs, such as loss of adhesion between cells, nucleus fragmentation, necrosis, and hemorrhage. Samples showed signs of recovery but severe damages were still observed. Neither HPLC-PDA nor mass spectrometry analysis showed any evidence of free Microcystins bioaccumulation.

Citing Articles

Harmful Algal Bloom Toxicity in Tadpoles.

Su R, Meyers C, Warner E, Garcia J, Refsnider J, Lad A Toxins (Basel). 2020; 12(6).

PMID: 32521650 PMC: 7354472. DOI: 10.3390/toxins12060378.

References

Rymuszka A . Microcystin-LR induces cytotoxicity and affects carp immune cells by impairment of their phagocytosis and the organization of the cytoskeleton. J Appl Toxicol. 2012; 33(11):1294-302. DOI: 10.1002/jat.2791. View

Ding W, Shen H, Ong C . Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes. J Toxicol Environ Health A. 2001; 64(6):507-19. DOI: 10.1080/152873901753215966. View

FISCHER , Dietrich . Toxicity of the cyanobacterial cyclic heptapeptide toxins microcystin-LR and -RR in early life-stages of the African clawed frog (Xenopus laevis). Aquat Toxicol. 2000; 49(3):189-198. DOI: 10.1016/s0166-445x(99)00079-x. View

Goldberg J, Huang H, Kwon Y, Greengard P, Nairn A, Kuriyan J . Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1. Nature. 1995; 376(6543):745-53. DOI: 10.1038/376745a0. View

Ferreira M, Oliveira V, Oliveira R, da Cunha P, Grisolia C, Pires Jr O . Histopathological effects of [D-Leu(1)]Microcystin-LR variants on liver, skeletal muscle and intestinal tract of Hypophthalmichthys molitrix (Valenciennes, 1844). Toxicon. 2010; 55(7):1255-62. DOI: 10.1016/j.toxicon.2010.01.016. View

Jacquet C, Thermes V, de Luze A, Puiseux-Dao S, Bernard C, Joly J . Effects of microcystin-LR on development of medaka fish embryos (Oryzias latipes). Toxicon. 2004; 43(2):141-7. DOI: 10.1016/j.toxicon.2003.11.010. View

Chen D, Zeng J, Wang F, Zheng W, Tu W, Zhao J . Hyperphosphorylation of intermediate filament proteins is involved in microcystin-LR-induced toxicity in HL7702 cells. Toxicol Lett. 2012; 214(2):192-9. DOI: 10.1016/j.toxlet.2012.08.024. View

Neffling M, Lance E, Meriluoto J . Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry. Environ Pollut. 2009; 158(3):948-52. DOI: 10.1016/j.envpol.2009.10.023. View

Eriksson J, Paatero G, Meriluoto J, Codd G, Kass G, Nicotera P . Rapid microfilament reorganization induced in isolated rat hepatocytes by microcystin-LR, a cyclic peptide toxin. Exp Cell Res. 1989; 185(1):86-100. DOI: 10.1016/0014-4827(89)90039-6. View

10.

Bagu J, Sykes B, CRAIG M, Holmes C . A molecular basis for different interactions of marine toxins with protein phosphatase-1. Molecular models for bound motuporin, microcystins, okadaic acid, and calyculin A. J Biol Chem. 1997; 272(8):5087-97. DOI: 10.1074/jbc.272.8.5087. View

11.

Runnegar M, Berndt N, Kong S, Lee E, Zhang L . In vivo and in vitro binding of microcystin to protein phosphatases 1 and 2A. Biochem Biophys Res Commun. 1995; 216(1):162-9. DOI: 10.1006/bbrc.1995.2605. View

12.

van Halderen A, Harding W, Wessels J, Schneider D, HEINE E, Van der Merwe J . Cyanobacterial (blue-green algae) poisoning of livestock in the western Cape Province of South Africa. J S Afr Vet Assoc. 1995; 66(4):260-4. View

13.

Zhou M, Tu W, Xu J . Mechanisms of microcystin-LR-induced cytoskeletal disruption in animal cells. Toxicon. 2015; 101:92-100. DOI: 10.1016/j.toxicon.2015.05.005. View

14.

Frangez R, Zuzek M, Mrkun J, Suput D, Sedmak B, Kosec M . Microcystin-LR affects cytoskeleton and morphology of rabbit primary whole embryo cultured cells in vitro. Toxicon. 2003; 41(8):999-1005. DOI: 10.1016/s0041-0101(03)00072-2. View

15.

Khan S, Wickstrom M, Haschek W, Schaeffer D, Ghosh S, Beasley V . Microcystin-LR and kinetics of cytoskeletal reorganization in hepatocytes, kidney cells, and fibroblasts. Nat Toxins. 1996; 4(5):206-14. DOI: 10.1002/(SICI)(1996)4:5<206::AID-NT2>3.0.CO;2-X. View

16.

Batista T, de Sousa G, Suput J, Rahmani R, Suput D . Microcystin-LR causes the collapse of actin filaments in primary human hepatocytes. Aquat Toxicol. 2003; 65(1):85-91. DOI: 10.1016/s0166-445x(03)00108-5. View

17.

Espina B, Louzao M, Cagide E, Alfonso A, Vieytes M, Yasumoto T . The methyl ester of okadaic acid is more potent than okadaic acid in disrupting the actin cytoskeleton and metabolism of primary cultured hepatocytes. Br J Pharmacol. 2009; 159(2):337-44. PMC: 2825355. DOI: 10.1111/j.1476-5381.2009.00512.x. View

18.

Wickstrom M, Khan S, Haschek W, Wyman J, Eriksson J, Schaeffer D . Alterations in microtubules, intermediate filaments, and microfilaments induced by microcystin-LR in cultured cells. Toxicol Pathol. 1995; 23(3):326-37. DOI: 10.1177/019262339502300309. View

19.

Eriksson J, Gronberg L, Nygard S, Slotte J, Meriluoto J . Hepatocellular uptake of 3H-dihydromicrocystin-LR, a cyclic peptide toxin. Biochim Biophys Acta. 1990; 1025(1):60-6. DOI: 10.1016/0005-2736(90)90190-y. View

20.

Dvorakova D, Dvorakova K, Blaha L, Marsalek B, Knotkova Z . Effects of cyanobacterial biomass and purified microcystins on malformations in Xenopus laevis: teratogenesis assay (FETAX). Environ Toxicol. 2002; 17(6):547-55. DOI: 10.1002/tox.10088. View