OMIP-048 MC: Quantification of Calcium Sensors and Channels Expression in Lymphocyte Subsets by Mass Cytometry

Calcium (Ca ) signaling controls T-cell activation and functions. Ca concentrations are locally detected and controlled by Ca -sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca -channels (ORAI1-3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca -sensors and -channels in human and murine cells, and further devised a 18-antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets.