Identification of the Pleiotropic SacQ Gene of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1986 Apr 1

PMID 3007431

Citations 40

Authors

M Yang

E Ferrari

E Chen

D J Henner

Affiliations

Soon will be listed here.

Abstract

The sacQ gene of Bacillus subtilis, a pleiotropic gene affecting the expression of a number of secreted gene products, has been identified as a small 46-amino-acid polypeptide. The increased expression of this polypeptide in strains carrying the sacQ36 allele, or in strains carrying the sacQ gene on a high copy plasmid, appears to be responsible for the phenotype of higher levels of proteases seen in these strains. A deletion of the sacQ gene had no apparent phenotype, indicating that it is not an essential gene.

Citing Articles

Reversible conjugation of a CBASS nucleotide cyclase regulates bacterial immune response to phage infection.

Kruger L, Gaskell-Mew L, Graham S, Shirran S, Hertel R, White M Nat Microbiol. 2024; 9(6):1579-1592.

PMID: 38589469 PMC: 11153139. DOI: 10.1038/s41564-024-01670-5.

Expression of degQ gene and its effect on lipopeptide production as well as formation of secretory proteases in Bacillus subtilis strains.

Lilge L, Vahidinasab M, Adiek I, Becker P, Kuppusamy Nesamani C, Treinen C Microbiologyopen. 2021; 10(5):e1241.

PMID: 34713601 PMC: 8515880. DOI: 10.1002/mbo3.1241.

A DegU-P and DegQ-Dependent Regulatory Pathway for the K-state in .

Miras M, Dubnau D Front Microbiol. 2016; 7:1868.

PMID: 27920766 PMC: 5118428. DOI: 10.3389/fmicb.2016.01868.

The canonical twin-arginine translocase components are not required for secretion of folded green fluorescent protein from the ancestral strain of Bacillus subtilis.

Snyder A, Mukherjee S, Glass J, Kearns D, Mukhopadhyay S Appl Environ Microbiol. 2014; 80(10):3219-32.

PMID: 24632256 PMC: 4018911. DOI: 10.1128/AEM.00335-14.

Tracing the domestication of a biofilm-forming bacterium.

McLoon A, Guttenplan S, Kearns D, Kolter R, Losick R J Bacteriol. 2011; 193(8):2027-34.

PMID: 21278284 PMC: 3133032. DOI: 10.1128/JB.01542-10.

References

Aubert E, Klier A, Rapoport G . Cloning and expression in Escherichia coli of the regulatory sacU gene from Bacillus subtilis. J Bacteriol. 1985; 161(3):1182-7. PMC: 215024. DOI: 10.1128/jb.161.3.1182-1187.1985. View

Anagnostopoulos C, Spizizen J . REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961; 81(5):741-6. PMC: 279084. DOI: 10.1128/jb.81.5.741-746.1961. View

Mills D, Kramer F . Structure-independent nucleotide sequence analysis. Proc Natl Acad Sci U S A. 1979; 76(5):2232-5. PMC: 383572. DOI: 10.1073/pnas.76.5.2232. View

FERRARI F, Nguyen A, Lang D, Hoch J . Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983; 154(3):1513-5. PMC: 217638. DOI: 10.1128/jb.154.3.1513-1515.1983. View

Sekiguchi J, Takada N, Okada H . Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg. J Bacteriol. 1975; 121(2):688-94. PMC: 245983. DOI: 10.1128/jb.121.2.688-694.1975. View

Caulfield M, Horiuchi S, Tai P, Davis B . The 64-kilodalton membrane protein of Bacillus subtilis is also present as a multiprotein complex on membrane-free ribosomes. Proc Natl Acad Sci U S A. 1984; 81(24):7772-6. PMC: 392234. DOI: 10.1073/pnas.81.24.7772. View

Kunst F, Pascal M, Lepesant J, Billault A, DEDONDER R . Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168. Biochimie. 1974; 56(11-12):1481-9. DOI: 10.1016/s0300-9084(75)80270-7. View

Murray C, RABINOWITZ J . Nucleotide sequences of transcription and translation initiation regions in Bacillus phage phi 29 early genes. J Biol Chem. 1982; 257(2):1053-62. View

Priest F . Extracellular enzyme synthesis in the genus Bacillus. Bacteriol Rev. 1977; 41(3):711-53. PMC: 414021. DOI: 10.1128/br.41.3.711-753.1977. View

10.

Hoch J, Barat M, Anagnostopoulos C . Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967; 93(6):1925-37. PMC: 276712. DOI: 10.1128/jb.93.6.1925-1937.1967. View

11.

Aiba H, Adhya S, de Crombrugghe B . Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem. 1981; 256(22):11905-10. View

12.

Band L, Henner D . Bacillus subtilis requires a "stringent" Shine-Dalgarno region for gene expression. DNA. 1984; 3(1):17-21. DOI: 10.1089/dna.1.1984.3.17. View

13.

Maniatis T, Jeffrey A, Kleid D . Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975; 72(3):1184-8. PMC: 432491. DOI: 10.1073/pnas.72.3.1184. View

14.

Horinouchi S, Weisblum B . Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982; 150(2):815-25. PMC: 216434. DOI: 10.1128/jb.150.2.815-825.1982. View

15.

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

16.

Ito K, Wittekind M, Nomura M, Shiba K, Yura T, Miura A . A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins. Cell. 1983; 32(3):789-97. DOI: 10.1016/0092-8674(83)90065-x. View

17.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

18.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

19.

Zuber P, Losick R . Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation. Cell. 1983; 35(1):275-83. DOI: 10.1016/0092-8674(83)90230-1. View

20.

McLaughlin J, Murray C, RABINOWITZ J . Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. J Biol Chem. 1981; 256(21):11283-91. View