Histone Methyltransferase MRNA Levels Increase in Response to Curative Hormone Treatment for Cryptorchidism-Dependent Male Infertility

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2018 Aug 4

PMID 30071651

Citations 5

Authors

Faruk Hadziselimovic

Gieri Cathomas

Gilvydas Verkauskas

Darius Dasevicius

Michael B Stadler

Affiliations

Soon will be listed here.

Abstract

There is a correlation between cryptorchidism and an increased risk of testicular cancer and infertility. During orchidopexy, testicular biopsies are performed to confirm the presence of type A dark (Ad) spermatogonia, which are a marker for low infertility risk (LIR). The Ad spermatogonia are absent in high infertility risk (HIR) patients, who are treated with a gonadotropin-releasing hormone agonist (GnRHa) to significantly lower the risk of infertility. Despite its prevalence, little is known about the molecular events involved in cryptorchidism. Previously, we compared the transcriptomes of LIR versus HIR patients treated with and without hormones. Here, we interpreted data regarding members of the positive regulatory domain-containing () family; some of which encoded histone methyltransferases that are important for reproduction. We found there were lower levels of , , , , and mRNA in the testes of HIR patients compared with LIR patients, and that , , and were significantly induced after GnRHa treatment. Furthermore, we observed protein staining in the cytoplasm of germ cells in the testes from LIR and HIR patients, indicating that the mRNA and protein levels corresponded. This result indicated that the curative hormonal therapy for cryptorchidism involved conserved chromatin modification enzymes.

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