Both DNA Topoisomerases I and II Relax 2 Micron Plasmid DNA in Living Yeast Cells

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 1986 Apr 11

PMID 3006927

Citations 38

Authors

R A Saavedra

J A Huberman

Affiliations

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Abstract

Measurements at various temperatures of the linking number of yeast 2 microns plasmid DNA in wild-type cells and in cells bearing mutations in the DNA topoisomerase I and II genes show that bulk 2 microns plasmid minichromosome are maintained in a relaxed state by the combined action of topoisomerases I and II. Bulk 2 microns minichromosomes are not under torsional stress in vivo and are not substrates for a putative gyrase-like topoisomerase.

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