Identification and Characterisation of PmaCI an Endonuclease of Novel Specificity from Pseudomonas Maltophila

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Feb 11

PMID 3005970

Citations 7

Authors

J N Walker

P D Dean

J R Saunders

Affiliations

Soon will be listed here.

Abstract

We report the use of MonoQ FPLC (Fast Protein Liquid Chromatography) for the rapid purification of a novel Type II restriction endonuclease PmaCI, from Pseudomonas maltophila, which recognises the sequence 5'-CAC decreases GTG-3'. The resulting enzyme is free of other nucleases to a level suitable for its characterisation by multiple-substrate digestion and DNA sequencing techniques. This method appears to be widely applicable and we have used it for the isolation of restriction endonucleases of comparable purity from a range of other organisms. Also described is a rapid method for screening a library of small inserted regions in recombinant M13 molecules for the presence and subsequent screening of restriction sites of interest.

Citing Articles

Mechanism of intramolecular recyclization and deletion formation following transformation of Escherichia coli with linearized plasmid DNA.

Conley E, Saunders V, Jackson V, Saunders J Nucleic Acids Res. 1986; 14(22):8919-32.

PMID: 3537964 PMC: 311920. DOI: 10.1093/nar/14.22.8919.

Restriction enzymes and their isoschizomers.

Roberts R Nucleic Acids Res. 1987; 15 Suppl:r189-217.

PMID: 3033611 PMC: 339886. DOI: 10.1093/nar/15.suppl.r189.

Sequence analysis of the NgoPII methyltransferase gene from Neisseria gonorrhoeae P9: homologies with other enzymes recognizing the sequence 5'-GGCC-3'.

Sullivan K, Saunders J Nucleic Acids Res. 1988; 16(10):4369-87.

PMID: 2837733 PMC: 336636. DOI: 10.1093/nar/16.10.4369.

Restriction enzymes and their isoschizomers.

Roberts R Nucleic Acids Res. 1988; 16 Suppl:r271-313.

PMID: 2835753 PMC: 340913. DOI: 10.1093/nar/16.suppl.r271.

Restriction enzymes and their isoschizomers.

Roberts R Nucleic Acids Res. 1989; 17 Suppl:r347-87.

PMID: 2541417 PMC: 334787. DOI: 10.1093/nar/17.suppl.r347.

References

Smith H, WILCOX K . A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970; 51(2):379-91. DOI: 10.1016/0022-2836(70)90149-x. View

Smith H, Nathans D . Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973; 81(3):419-23. DOI: 10.1016/0022-2836(73)90152-6. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Roberts R . Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1985; 13 Suppl:r165-200. PMC: 320508. DOI: 10.1093/nar/13.suppl.r165. View

Mount D, Conrad B . Microcomputer programs for graphic analysis of nucleic acid and protein sequences. Nucleic Acids Res. 1984; 12(1 Pt 2):811-7. PMC: 321095. DOI: 10.1093/nar/12.1part2.811. View