Deuterium Oxide (D2O) Enhances the Photosensitivity of Stentor Coeruleus

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1985 Dec 1

PMID 3004613

Authors

K Iwatsuki

P S Song

Affiliations

Soon will be listed here.

Abstract

Stentor coeruleus exhibits negative phototaxis and step-up photophobic response (avoiding reaction) to visible light (maximum at 610-620 nm in both responses). In the presence of deuterium oxide (D2O) the step-up photophobic response was markedly enhanced, whereas the phototactic orientation response was inhibited. The induction time for the step-up photophobic response was longer in D2O than in H2O, and the duration of ciliary reversal for the response was also longer in D2O than in H2O, indicating that certain steps of the sensory transduction chain are subject to solvent deuterium isotope effects. The enhancement of the step-up photophobic response in D2O was canceled by LaCl3, while the inhibition of the phototactic orientation response in D2O was partially removed by LaCl3, even though LaCl3 did not affect the phototactic orientation response. These results suggest that the sensory transduction mechanisms for the two photoresponses are different, although the photoreceptors (stentorin) are the same.

References

Naitoh Y, Eckert R . Ionic mechanisms controlling behavioral responses of paramecium to mechanical stimulation. Science. 1969; 164(3882):963-5. DOI: 10.1126/science.164.3882.963. View

KAMINER B, Kimura J . Deuterium oxide: inhibition of calcium release in muscle. Science. 1972; 176(4033):406-7. DOI: 10.1126/science.176.4033.406. View

Naitoh Y, Eckert R, Friedman K . A regenerative calcium response in Paramecium. J Exp Biol. 1972; 56(3):667-81. DOI: 10.1242/jeb.56.3.667. View

Wood D . Action spectrum and electrophysiological responses correlated with the photophobic response of Stentor coeruleus. Photochem Photobiol. 1976; 24(3):261-6. DOI: 10.1111/j.1751-1097.1976.tb06821.x. View

Walker E, Lee T, Song P . Spectroscopic characterization of the Stentor photoreceptor. Biochim Biophys Acta. 1979; 587(1):129-44. DOI: 10.1016/0304-4165(79)90227-7. View

PETRAKIS N, Davis M, Siegel B . DISTURBANCE IN THE PROLIFERATION AND DIFFERENTIATION OF MONONUCLEAR LEUCOCYTES TO FIBROBLASTS IN DIFFUSION CHAMBERS BY DEUTERIUM OXIDE. Nature. 1963; 200:591-2. DOI: 10.1038/200591a0. View

Song P, Walker E, Auerbach R, Robinson G . Proton release from Stentor photoreceptors in the excited states. Biophys J. 1981; 35(2):551-5. PMC: 1327544. DOI: 10.1016/S0006-3495(81)84811-4. View

Cecchi G, Colomo F, Lombardi V . Force-velocity relation in deuterium oxide-treated frog single muscle fibres during the rise of tension in an isometric tetanus. J Physiol. 1981; 317:207-21. PMC: 1246785. DOI: 10.1113/jphysiol.1981.sp013821. View

Kim I, Prusti R, Song P, Hader D, Hader M . Phototaxis and photophobic responses in Stentor coeruleus. Action spectrum and role of Ca2+ fluxes. Biochim Biophys Acta. 1984; 799(3):298-304. DOI: 10.1016/0304-4165(84)90274-5. View

10.

Prusti R, Song P, Hader D, Hader M . Caffeine-enhanced photomovement in the ciliate, Stentor coeruleus. Photochem Photobiol. 1984; 40(3):369-75. DOI: 10.1111/j.1751-1097.1984.tb04600.x. View

11.

Walker E, Yoon M, Song P . The pH dependence of photosensory responses in Stentor coeruleus and model system. Biochim Biophys Acta. 1981; 634(2):289-308. DOI: 10.1016/0005-2728(81)90148-1. View