Haemophilus Parasuis α-2,3-sialyltransferase-mediated Lipooligosaccharide Sialylation Contributes to Bacterial Pathogenicity

Overview

Journal Virulence

Specialty Microbiology

Date 2018 Jul 24

PMID 30036124

Citations 10

Authors

Huan Wang

Lu Liu

Qi Cao

Weiting Mao

Yage Zhang

Xinyi Qu

Xuwang Cai

Yujin Lv

Huanchun Chen

Xiaojuan Xu

Xiangru Wang

Affiliations

Soon will be listed here.

Abstract

Bacterial lipooligosaccharide (LOS) is an important virulence-associated factor, and its sialylation largely confers its ability to mediate cell adhesion, invasion, inflammation, and immune evasion. Here, we investigated the function of the Haemophilus parasuis α-2,3-sialyltransferase gene, lsgB, which determines the terminal sialylation of LOS, by generating a lsgB deletion mutant as well as a complementation strain. Our data indicate a direct effect of lsgB on LOS sialylation and reveal important roles of lsgB in promoting the pathogenicity of H. parasuis, including adhesion to and invasion of porcine cells in vitro, bacterial load and survival in vivo, as well as a contribution to serum resistance. These observations highlight the function of lsgB in mediating LOS sialylation and more importantly its role in H. parasuis infection. These findings provide a more profound understanding of the pathogenic mechanism of this disease-causing bacterium.

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