The Prognostic Value of CD8 and CD45RO T Cells Infiltration and Beclin1 Expression Levels for Early Postoperative Cholangitis of Biliary Atresia Patients After Kasai Operation

Overview

Journal J Korean Med Sci

Specialty General Medicine

Date 2018 Jul 24

PMID 30034306

Citations 6

Authors

Hong Jiang

Pengfei Gao

Huadong Chen

Zhihai Zhong

Man Shu

Zhichong Zhang

Jinbiao She

Juncheng Liu

Affiliations

Soon will be listed here.

Abstract

Background: Postoperative cholangitis is a common but severe complication after Kasai portoenterostomy for biliary atresia (BA). This study aimed to identify its prognostic factors.

Methods: Two sets of liver paraffin-embedded tissue samples were collected from BA patients who received Kasai portoenterostomy (n = 25 and n = 31, respectively). Patients were divided into non-cholangitis and cholangitis groups. The infiltration of CD4, CD8, CD45RO, CD68 cells and expression of Beclin1 were quantitatively evaluated in immunohistochemical analysis.

Results: Cholangitis group had a significantly lower CD8 T cell infiltration but a higher CD45RO cell infiltration, and a lower Beclin1 level than non-cholangitis group (all < 0.01). Multivariate logistic regression analysis indicated that infiltration of CD8 cells (odds ratio [OR], 0.112; 95% confidence interval [CI], 0.022-0.577) and CD45RO cells (OR, 3.88; 95% CI, 1.37-11.03), and Beclin1 level (OR, 0.088; 95% CI, 0.018-0.452) were independent influence factors for early postoperative cholangitis. Receiver operating characteristic (ROC) analysis showed that area under ROC curve (AUROC) values for CD8 cells, CD45RO cells and Beclin1 were 0.857, 0.738 and 0.900, respectively.

Conclusion: Our findings demonstrated the CD8 cells, CD45RO cells and Beclin1 level possessed the prognostic value for early postoperative cholangitis following Kasai operation, which may be helpful to develop new prevention and treatment strategies for postoperative cholangitis.

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