Molecular Characterization and New Genotypes of Enterocytozoon Bieneusi in Minks (Neovison Vison) in China

Overview

Journal Parasite

Specialty Parasitology

Date 2018 Jul 21

PMID 30028674

Citations 7

Authors

Wei Cong

Si-Yuan Qin

Qing-Feng Meng

Affiliations

Soon will be listed here.

Abstract

Microsporidiosis is an emerging and opportunistic disease, and Enterocytozoon bieneusi is the main cause of this disease in humans. Little information is available on prevalence and genotyping of E. bieneusi in minks. We collected 559 feces samples of minks from Heilongjiang and Jilin provinces in 2017, and studied E. bieneusi prevalence by nested PCR. A total of 23 out of 559 minks (4.1%) were detected as E. bieneusi-positive, and were raised in five of the seven investigated farms. Age was the only risk factor associated with E. bieneusi prevalence in investigated minks through logistic regression analysis. Sequence analysis of the ITS gene revealed that five E. bieneusi ITS genotypes, including Peru11, EbpC, and three novel genotypes (HLJM-1, HLJM-2 and JLM-1) were present, suggesting minks may be a potential source of human microsporidiosis.

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References

Xu C, Ma X, Zhang H, Zhang X, Zhao J, Ba H . Prevalence, risk factors and molecular characterization of Enterocytozoon bieneusi in raccoon dogs (Nyctereutes procyonoides) in five provinces of Northern China. Acta Trop. 2016; 161:68-72. DOI: 10.1016/j.actatropica.2016.05.015. View

DESPORTES I, Le Charpentier Y, GALIAN A, Bernard F, Cochand-Priollet B, Lavergne A . Occurrence of a new microsporidan: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS. J Protozool. 1985; 32(2):250-4. DOI: 10.1111/j.1550-7408.1985.tb03046.x. View

Li W, Deng L, Yu X, Zhong Z, Wang Q, Liu X . Multilocus genotypes and broad host-range of Enterocytozoon bieneusi in captive wildlife at zoological gardens in China. Parasit Vectors. 2016; 9(1):395. PMC: 4939065. DOI: 10.1186/s13071-016-1668-1. View

Zhang X, Cong W, Lou Z, Ma J, Zheng W, Yao Q . Prevalence, risk factors and multilocus genotyping of Enterocytozoon bieneusi in farmed foxes (Vulpes lagopus), Northern China. Parasit Vectors. 2016; 9:72. PMC: 4743323. DOI: 10.1186/s13071-016-1356-1. View

Yu F, Wu Y, Li T, Cao J, Wang J, Hu S . High prevalence of Enterocytozoon bieneusi zoonotic genotype D in captive golden snub-nosed monkey (Rhinopithecus roxellanae) in zoos in China. BMC Vet Res. 2017; 13(1):158. PMC: 5460354. DOI: 10.1186/s12917-017-1084-6. View

Wang L, Xiao L, Duan L, Ye J, Guo Y, Guo M . Concurrent infections of Giardia duodenalis, Enterocytozoon bieneusi, and Clostridium difficile in children during a cryptosporidiosis outbreak in a pediatric hospital in China. PLoS Negl Trop Dis. 2013; 7(9):e2437. PMC: 3772047. DOI: 10.1371/journal.pntd.0002437. View

Prieto A, Fernandez-Antonio R, Diaz-Cao J, Lopez G, Diaz P, Alonso J . Distribution of Aleutian mink disease virus contamination in the environment of infected mink farms. Vet Microbiol. 2017; 204:59-63. DOI: 10.1016/j.vetmic.2017.04.013. View

Zhang X, Shi W, Zhang N, Shi K, Li J, Xu P . Prevalence and genetic characterization of Toxoplasma gondii in donkeys in northeastern China. Infect Genet Evol. 2017; 54:455-457. DOI: 10.1016/j.meegid.2017.08.008. View

Zheng W, Zhang X, Ma J, Li F, Zhao Q, Huang S . Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP. PLoS One. 2016; 11(11):e0165308. PMC: 5091863. DOI: 10.1371/journal.pone.0165308. View

10.

Didier E . Microsporidiosis: an emerging and opportunistic infection in humans and animals. Acta Trop. 2005; 94(1):61-76. DOI: 10.1016/j.actatropica.2005.01.010. View

11.

Li W, Song Y, Zhong Z, Huang X, Wang C, Li C . Population genetics of Enterocytozoon bieneusi in captive giant pandas of China. Parasit Vectors. 2017; 10(1):499. PMC: 5648467. DOI: 10.1186/s13071-017-2459-z. View

12.

Karim M, Wang R, Dong H, Zhang L, Li J, Zhang S . Genetic polymorphism and zoonotic potential of Enterocytozoon bieneusi from nonhuman primates in China. Appl Environ Microbiol. 2014; 80(6):1893-8. PMC: 3957649. DOI: 10.1128/AEM.03845-13. View

13.

Ma J, Li P, Zhao X, Xu H, Wu W, Wang Y . Occurrence and molecular characterization of Cryptosporidium spp. and Enterocytozoon bieneusi in dairy cattle, beef cattle and water buffaloes in China. Vet Parasitol. 2014; 207(3-4):220-7. DOI: 10.1016/j.vetpar.2014.10.011. View

14.

Thellier M, Breton J . Enterocytozoon bieneusi in human and animals, focus on laboratory identification and molecular epidemiology. Parasite. 2008; 15(3):349-58. DOI: 10.1051/parasite/2008153349. View

15.

Deng L, Li W, Zhong Z, Gong C, Cao X, Song Y . Multi-locus genotypes of Enterocytozoon bieneusi in captive Asiatic black bears in southwestern China: High genetic diversity, broad host range, and zoonotic potential. PLoS One. 2017; 12(2):e0171772. PMC: 5300288. DOI: 10.1371/journal.pone.0171772. View

16.

Hu Y, Feng Y, Huang C, Xiao L . Occurrence, source, and human infection potential of Cryptosporidium and Enterocytozoon bieneusi in drinking source water in Shanghai, China, during a pig carcass disposal incident. Environ Sci Technol. 2014; 48(24):14219-27. PMC: 5788171. DOI: 10.1021/es504464t. View

17.

Jiang Y, Tao W, Wan Q, Li Q, Yang Y, Lin Y . Zoonotic and Potentially Host-Adapted Enterocytozoon bieneusi Genotypes in Sheep and Cattle in Northeast China and an Increasing Concern about the Zoonotic Importance of Previously Considered Ruminant-Adapted Genotypes. Appl Environ Microbiol. 2015; 81(10):3326-35. PMC: 4407225. DOI: 10.1128/AEM.00328-15. View

18.

Karim M, Yu F, Li J, Li J, Zhang L, Wang R . First molecular characterization of enteric protozoa and the human pathogenic microsporidian, Enterocytozoon bieneusi, in captive snakes in China. Parasitol Res. 2014; 113(8):3041-8. DOI: 10.1007/s00436-014-3967-9. View

19.

Qi M, Luo N, Wang H, Yu F, Wang R, Huang J . Zoonotic Cryptosporidium spp. and Enterocytozoon bieneusi in pet chinchillas (Chinchilla lanigera) in China. Parasitol Int. 2015; 64(5):339-41. DOI: 10.1016/j.parint.2015.05.007. View

20.

Yang Z, Zhao W, Shen Y, Zhang W, Shi Y, Ren G . Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China. Parasite. 2016; 23:52. PMC: 5134669. DOI: 10.1051/parasite/2016063. View