Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination Between Zika and Dengue Viruses

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2018 Jul 20

PMID 30023708

Citations 18

Authors

Marjorie C L C Freire

Laercio Pol-Fachin

Danilo F Coelho

Isabelle F T Viana

Tereza Magalhaes

Marli T Cordeiro

Nico Fischer

Felix F Loeffler

Thomas Jaenisch

Rafael F Franca

Ernesto T A Marques

Roberto D Lins

Affiliations

Soon will be listed here.

Abstract

B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% between the NS1 from ZIKV and the four dengue virus (DENV) serotypes, epitope sequences were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for a dramatically divergent electrostatic surface potential on the epitope regions of ZIKV and DENV2 serotypes. These findings suggest that strategies for differential diagnostics on the basis of short linear NS1 sequences are likely to fail due to immunological cross-reactions. Overall, results provide the molecular basis of differential discrimination between Zika and DENVs by NS1 monoclonal antibodies.

Citing Articles

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Assessing West Nile virus (WNV) and Usutu virus (USUV) exposure in bird ringers in the Netherlands: a high-risk group for WNV and USUV infection?.

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Muniz A, Tolesano-Pascoli G, Vieira R, Polli M, da Silva Rodrigues V, Gonzaga H Exp Appl Acarol. 2023; 89(2):317-327.

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Differential critical residues on the overlapped region of the non-structural protein-1 recognized by flavivirus and dengue virus cross-reactive monoclonal antibodies.

Luangaram P, Tamdet C, Saengwong C, Prommool T, Kraivong R, Nilchan N Sci Rep. 2022; 12(1):21548.

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