Leptin Stimulates Tissue Rat Mast Cell Pro-inflammatory Activity and Migratory Response

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2018 Jul 19

PMID 30019195

Citations 11

Authors

Paulina Zelechowska

Justyna Agier

Sylwia Rozalska

Magdalena Wiktorska

Ewa Brzezinska-Blaszczyk

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to determine whether leptin, a member of the adipocytokines involved in immune and inflammatory response regulation, may influence some aspects of mast cell biology.

Materials And Methods: Experiments were done in vitro on fully mature tissue rat mast cells isolated from the peritoneal cavity, and leptin was used at concentrations 0.001-100 ng/ml. The effect of leptin on mast cell degranulation (histamine release assay), intracellular Ca level (fluorimetry), pro-inflammatory mediator release (ELISA technique), surface receptor expression (flow cytometry and confocal microscopy), and migration (Boyden microchamber assay) was estimated.

Results: Leptin was found to stimulate mast cells to degranulation and histamine release. It induced the intracellular Ca increase, as well. In response to leptin stimulation, mast cells generated and released cysLTs and chemokine CCL3. Leptin-induced upregulation of CYSLTR1 and CYSLTR2 surface expression was observed. Moreover, this adipocytokine stimulated mast cells to migratory response, even in the absence of extracellular matrix (ECM) proteins.

Conclusions: Our observations clearly documented that leptin promotes the pro-inflammatory activity of mast cells, and it thereby engages these cells in the inflammatory processes.

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