A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use As a Bone Graft Substitute: A Critical Evaluation of Processing and Structure

Overview

Journal J Funct Biomater

Specialty Biomedical Engineering

Date 2018 Jul 14

PMID 30002336

Citations 28

Authors

Daniel N Bracey

Thorsten M Seyler

Alexander H Jinnah

Mark O Lively

Jeffrey S Willey

Thomas L Smith

Mark E Van Dyke

Patrick W Whitlock

Affiliations

Soon will be listed here.

Abstract

Background: Bone grafts are used in approximately one half of all musculoskeletal surgeries. Autograft bone is the historic gold standard but is limited in supply and its harvest imparts significant morbidity to the patient. Alternative sources of bone graft include allografts, synthetics and, less commonly, xenografts which are taken from animal species. Xenografts are available in unlimited supply from healthy animal donors with controlled biology, avoiding the risk of human disease transmission, and may satisfy current demand for bone graft products.

Methods: In the current study, cancellous bone was harvested from porcine femurs and subjected to a novel decellularization protocol to derive a bone scaffold.

Results: The scaffold was devoid of donor cellular material on histology and DNA sampling ( < 0.01). Microarchitectural properties important for osteoconductive potential were preserved after decellularization as shown by high resolution imaging modalities. Proteomics data demonstrated similar profiles when comparing the porcine bone scaffold against commercially available human demineralized bone matrix approved for clinical use.

Conclusion: We are unaware of any porcine-derived bone graft products currently used in orthopaedic surgery practice. Results from the current study suggest that porcine-derived bone scaffolds warrant further consideration to serve as a potential bone graft substitute.

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