Providing Reducing Power by Microalgal Photosynthesis: a Novel Perspective Towards Sustainable Biocatalytic Production of Bulk Chemicals Exemplified for Aliphatic Amines

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jul 12

PMID 29993023

Citations 5

Authors

Jana Lowe

Arthur Siewert

Anna-Catharina Scholpp

Lutz Wobbe

Harald Groger

Affiliations

Soon will be listed here.

Abstract

A biotechnological process is reported, which enables an enzymatic reduction without the need for addition of an organic co-substrate for in situ-cofactor recycling. The process is based on merging the fields of enzymatic reductive amination with formate dehydrogenase-based in situ-cofactor recycling and algae biotechnology by means of the photoautotrophic microorganism Chlamydomonas reinhardtii, providing the needed formate in situ by formation from carbon dioxide, water and light. This biotransformation has been exemplified for the synthesis of various aliphatic amines known as bulk chemicals.

Citing Articles

Expression and activity of heterologous hydroxyisocaproate dehydrogenases in sp. PCC 6803 Δ.

Jurkas V, Winkler C, Poschenrieder S, Oliveira P, Pacheco C, Ferreira E Eng Microbiol. 2024; 2(1):100008.

PMID: 39628613 PMC: 11610949. DOI: 10.1016/j.engmic.2021.100008.

Transmembrane Shuttling of Photosynthetically Produced Electrons to Propel Extracellular Biocatalytic Redox Reactions in a Modular Fashion.

Jurkas V, Weissensteiner F, De Santis P, Vrabl S, Sorgenfrei F, Bierbaumer S Angew Chem Weinheim Bergstr Ger. 2024; 134(40):e202207971.

PMID: 38505002 PMC: 10946770. DOI: 10.1002/ange.202207971.

Photobiocatalytic Strategies for Organic Synthesis.

Emmanuel M, Bender S, Bilodeau C, Carceller J, DeHovitz J, Fu H Chem Rev. 2023; 123(9):5459-5520.

PMID: 37115521 PMC: 10905417. DOI: 10.1021/acs.chemrev.2c00767.

Transmembrane Shuttling of Photosynthetically Produced Electrons to Propel Extracellular Biocatalytic Redox Reactions in a Modular Fashion.

Jurkas V, Weissensteiner F, De Santis P, Vrabl S, Sorgenfrei F, Bierbaumer S Angew Chem Int Ed Engl. 2022; 61(40):e202207971.

PMID: 35921249 PMC: 9804152. DOI: 10.1002/anie.202207971.

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.

Theodosiou E, Tullinghoff A, Toepel J, Buhler B Front Bioeng Biotechnol. 2022; 10:855715.

PMID: 35497353 PMC: 9043136. DOI: 10.3389/fbioe.2022.855715.

References

Mutti F, Knaus T, Scrutton N, Breuer M, Turner N . Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades. Science. 2015; 349(6255):1525-9. PMC: 4883652. DOI: 10.1126/science.aac9283. View

Wobbe L, Remacle C . Improving the sunlight-to-biomass conversion efficiency in microalgal biofactories. J Biotechnol. 2014; 201:28-42. DOI: 10.1016/j.jbiotec.2014.08.021. View

Mus F, Dubini A, Seibert M, Posewitz M, Grossman A . Anaerobic acclimation in Chlamydomonas reinhardtii: anoxic gene expression, hydrogenase induction, and metabolic pathways. J Biol Chem. 2007; 282(35):25475-86. DOI: 10.1074/jbc.M701415200. View

Gfeller R, Gibbs M . Fermentative Metabolism of Chlamydomonas reinhardtii: I. Analysis of Fermentative Products from Starch in Dark and Light. Plant Physiol. 1984; 75(1):212-8. PMC: 1066864. DOI: 10.1104/pp.75.1.212. View

Klassen V, Blifernez-Klassen O, Wobbe L, Schluter A, Kruse O, Mussgnug J . Efficiency and biotechnological aspects of biogas production from microalgal substrates. J Biotechnol. 2016; 234:7-26. DOI: 10.1016/j.jbiotec.2016.07.015. View

Bommarius B, Schurmann M, Bommarius A . A novel chimeric amine dehydrogenase shows altered substrate specificity compared to its parent enzymes. Chem Commun (Camb). 2014; 50(95):14953-5. DOI: 10.1039/c4cc06527a. View

Atteia A, van Lis R, Gelius-Dietrich G, Adrait A, Garin J, Joyard J . Pyruvate formate-lyase and a novel route of eukaryotic ATP synthesis in Chlamydomonas mitochondria. J Biol Chem. 2006; 281(15):9909-18. DOI: 10.1074/jbc.M507862200. View

Nakamura K, Yamanaka R . Light mediated cofactor recycling system in biocatalytic asymmetric reduction of ketone. Chem Commun (Camb). 2002; (16):1782-3. DOI: 10.1039/b203844g. View

Tishkov V, Popov V . Catalytic mechanism and application of formate dehydrogenase. Biochemistry (Mosc). 2005; 69(11):1252-67. DOI: 10.1007/s10541-005-0071-x. View

10.

Koninger K, Baraibar A, Mugge C, Paul C, Hollmann F, Nowaczyk M . Recombinant Cyanobacteria for the Asymmetric Reduction of C=C Bonds Fueled by the Biocatalytic Oxidation of Water. Angew Chem Int Ed Engl. 2016; 55(18):5582-5. DOI: 10.1002/anie.201601200. View

11.

Hoober J . The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use. Elizabeth H. Harris. Academic Press, San Diego, CA, 1989. xiv, 780 pp., illus. $145. Science. 1989; 246(4936):1503-4. DOI: 10.1126/science.246.4936.1503-a. View

12.

Abrahamson M, Vazquez-Figueroa E, Woodall N, Moore J, Bommarius A . Development of an amine dehydrogenase for synthesis of chiral amines. Angew Chem Int Ed Engl. 2012; 51(16):3969-72. DOI: 10.1002/anie.201107813. View

13.

Grossman A, Catalanotti C, Yang W, Dubini A, Magneschi L, Subramanian V . Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii. New Phytol. 2011; 190(2):279-88. DOI: 10.1111/j.1469-8137.2010.03534.x. View