Oxidative Degradation of Polyamines by Serum Supplement Causes Cytotoxicity on Cultured Cells

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jul 12

PMID 29991686

Citations 25

Authors

Linlin Wang

Ying Liu

Cui Qi

Luyao Shen

Junyan Wang

Xiangjun Liu

Nan Zhang

Tao Bing

Dihua Shangguan

Affiliations

Soon will be listed here.

Abstract

Serum is a common supplement for cell culture due to it containing the essential active components for the growth and maintenance of cells. However, the knowledges of the active components in serum are incomplete. Apart from the direct influence of serum components on cultured cells, the reaction of serum components with tested drugs cannot be ignored, which usually results in the false conclusion on the activity of the tested drugs. Here we report the toxicity effect of polyamines (spermidine and spermine) on cultured cells, especially on drug-resistant cancer cell lines, which resulted from the oxidative degradation of polyamines by amine oxidases in serum supplement. Upon adding spermidine or spermine, high concentration of HO an enzyme oxidation product of polyamines, was generated in culture media containing ruminant serum, such as fetal bovine serum (FBS), calf serum, bovine serum, goat serum or horse serum, but not in the media containing human serum. Drug-resistant cancer cell lines showed much higher sensitivity to the oxidation products of polyamines (HO and acrolein) than their wild cell lines, which was due to their low antioxidative capacity.

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