Responses of Biomarkers in Atlantic Salmon (Salmo Salar) Following Exposure to Environmentally Relevant Concentrations of Complex Metal Mixture (Zn, Cu, Ni, Cr, Pb, Cd). Part II

Overview

Journal Ecotoxicology

Specialties Environmental Health
Toxicology

Date 2018 Jul 11

PMID 29987536

Citations 3

Authors

Milda Stankeviciute

Gintare Sauliute

Tomas Makaras

Arvydas Markuckas

Tomas Virbickas

Janina Barsiene

Affiliations

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Abstract

The aim of this research was to assess interactions between metals at low exposure concentrations (Maximum-Permissible-Concentrations accepted for the inland waters in EU) and to assess possible influence of background exposure (10-times reduced concentration of a single metal) on toxicological significance of selected biomarkers in Salmo salar after treatment with metal mixture (Zn - 0.1, Cu - 0.01, Ni - 0.01, Cr - 0.01, Pb - 0.005 and Cd - 0.005 mg/L). The tissue-specific bioaccumulation, genotoxicity and cytotoxicity responses (erythrocytic nuclear abnormalities assay) in peripheral blood, kidneys, gills and liver erythrocytes of fish to metal mixtures were assessed after 14 days treatment. Treatment with primary mixture (MIX) or two variants of this mixture (Cr↓ (10 times reduced Cr concentration) and Cu↓ (10 times reduced Cu concentration)) induced the strongest responses in genotoxicity and cytotoxicity endpoints. Exposure to these mixtures highly affected Zn, Cu and Cd bioaccumulation in liver tissue. The highest amount of Ni accumulated was measured after Cd↓ treatment in all tissues. Treatments with reduced concentration of non-essential metal resulted in an increased accumulation of Pb, Ni, or Cd; treatments with reduced concentration of essential metal resulted in a reduced accumulation of certain metals (especially Cd and Pb) in tissues compared between treatments. Glucose content in blood and behavioural endpoints were evaluated after short-term exposure to metal mixtures (MIX, Cr↓, Cu↓). Significant increase in blood glucose concentration was measured after all treatments. These metal mixtures elicit significant behavioural alterations in fish. Consequently, this research revealed a significant influence of background exposure considering mixture toxicity.

Citing Articles

Response patterns of biomarkers in omnivorous and carnivorous fish species exposed to multicomponent metal (Cd, Cr, Cu, Ni, Pb and Zn) mixture. Part III.

Sauliute G, Markuckas A, Stankeviciute M Ecotoxicology. 2020; 29(3):258-274.

PMID: 32052347 DOI: 10.1007/s10646-020-02170-y.

Juvenile fish responses to sublethal leachate concentrations: comparison of sensitivity of different behavioral endpoints.

Makaras T, Montvydiene D, Kazlauskiene N, Stankeviciute M, Raudonyte-Svirbutaviciene E Environ Sci Pollut Res Int. 2019; 27(5):4876-4890.

PMID: 31845261 DOI: 10.1007/s11356-019-07211-6.

Genotoxic, cytotoxic, and neurotoxic responses in Anodonta cygnea after complex metal mixture treatment.

Butrimaviciene L, Stankeviciute M, Kalciene V, Joksas K, Barsiene J Environ Sci Pollut Res Int. 2019; 26(8):7627-7639.

PMID: 30666580 DOI: 10.1007/s11356-019-04206-1.

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