Establishment and Characterisation of Cell Lines from Patients with Lung Cancer (predominantly Small Cell Carcinoma)

Overview

Journal Br J Cancer

Specialty Oncology

Date 1985 Oct 1

PMID 2998422

Citations 31

Authors

P R Twentyman

N E Fox

G A Walls

P Workman

J.V. Watson

N Johnson

J G Reeve

N M Bleehen

Affiliations

Soon will be listed here.

Abstract

Tissue samples from 59 patients with lung cancer have been used to establish cell lines in culture. The primary diagnosis was small cell carcinoma in all except four. Most of the samples were of bone marrow but pleural effusions, lymph node biopsies and skin metastases were also included. The samples were usually split between HITES serum-free medium and HITES plus 2.5% foetal calf serum. A total of 19 cell lines were established and characterised. One line is large cell anaplastic lung carcinoma, four are B-lymphoblastoid and fourteen are small cell lung cancer. Considerable heterogeneity in gross morphology, neuroendocrine differentiation (by electron microscopy) and content of the enzyme L-dopa decarboxylase was seen. The use of HITES plus 2.5% foetal calf serum resulted in better establishment of cultures than did serum-free HITES.

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References

Walls G, Twentyman P . Cloning of human lung cancer cells. Br J Cancer. 1985; 52(4):505-13. PMC: 1977242. DOI: 10.1038/bjc.1985.221. View

Reeve J, Wulfrank D, Stewart J, Twentyman P, Bleehen N . Monoclonal-antibody-defined human lung tumour cell-surface antigens. Int J Cancer. 1985; 35(6):769-75. DOI: 10.1002/ijc.2910350612. View

Spurr A . A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969; 26(1):31-43. DOI: 10.1016/s0022-5320(69)90033-1. View

Anner R, Drewinko B . Frequency and significance of bone marrow involvement by metastatic solid tumors. Cancer. 1977; 39(3):1337-44. DOI: 10.1002/1097-0142(197703)39:3<1337::aid-cncr2820390349>3.0.co;2-x. View

BEAVEN M, Wilcox G, Terpstra G . A microprocedure for the measurement of 14CO2 release from [14C]carboxyl-labeled amino acids. Anal Biochem. 1978; 84(2):638-41. DOI: 10.1016/0003-2697(78)90089-1. View

COURTENAY V, Mills J . An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. Br J Cancer. 1978; 37(2):261-8. PMC: 2009601. DOI: 10.1038/bjc.1978.35. View

Baylin S, Stevens S, Shakir K . Association of diamine oxidase and ornithine decarboxylase with maturing cells in rapidly proliferating epithelium. Biochim Biophys Acta. 1978; 541(3):415-9. DOI: 10.1016/0304-4165(78)90200-3. View

Ihde D, Simms E, Matthews M, Cohen M, Bunn P, Minna J . Bone marrow metastases in small cell carcinoma of the lung: frequency, description, and influence on chemotherapeutic toxicity and prognosis. Blood. 1979; 53(4):677-86. View

Baylin S, Abeloff M, Goodwin G, Carney D, Gazdar A . Activities of L-dopa decarboxylase and diamine oxidase (histaminase) in human lung cancers and decarboxylase as a marker for small (oat) cell cancer in cell culture. Cancer Res. 1980; 40(6):1990-4. View

10.

TAYLOR I . A rapid single step staining technique for DNA analysis by flow microfluorimetry. J Histochem Cytochem. 1980; 28(9):1021-4. DOI: 10.1177/28.9.6157714. View

11.

Gazdar A, Carney D, RUSSELL E, Sims H, Baylin S, Bunn Jr P . Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 1980; 40(10):3502-7. View

12.

SORENSON G, Pettengill O, BRINCK-JOHNSEN T, Cate C, Maurer L . Hormone production by cultures of small-cell carcinoma of the lung. Cancer. 1981; 47(6):1289-96. DOI: 10.1002/1097-0142(19810315)47:6<1289::aid-cncr2820470610>3.0.co;2-b. View

13.

Gazdar A, Zweig M, Carney D, Van Steirteghen A, Baylin S, Minna J . Levels of creatine kinase and its BB isoenzyme in lung cancer specimens and cultures. Cancer Res. 1981; 41(7):2773-7. View

14.

Carney D, Bunn Jr P, Gazdar A, Pagan J, Minna J . Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung. Proc Natl Acad Sci U S A. 1981; 78(5):3185-9. PMC: 319525. DOI: 10.1073/pnas.78.5.3185. View

15.

Pettengill O, SORENSON G, CURPHEY T, Noll W, Cate C, Maurer L . Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer. 1980; 45(5):906-18. DOI: 10.1002/1097-0142(19800301)45:5<906::aid-cncr2820450513>3.0.co;2-h. View

16.

Watson J . Enzyme kinetic studies in cell populations using fluorogenic substrates and flow cytometric techniques. Cytometry. 1980; 1(2):143-51. DOI: 10.1002/cyto.990010209. View

17.

Moody T, Pert C, Gazdar A, Carney D, Minna J . High levels of intracellular bombesin characterize human small-cell lung carcinoma. Science. 1981; 214(4526):1246-8. DOI: 10.1126/science.6272398. View

18.

Whang-Peng J, Lee E, Bunn P, Carney D, Gazdar A, Minna J . Specific chromosome defect associated with human small-cell lung cancer; deletion 3p(14-23). Science. 1982; 215(4529):181-2. DOI: 10.1126/science.6274023. View

19.

Marangos P, Gazdar A, Carney D . Neuron specific enolase in human small cell carcinoma cultures. Cancer Lett. 1982; 15(1):67-71. DOI: 10.1016/0304-3835(82)90077-5. View

20.

Baylin S, Gazdar A, Minna J, Bernal S, Shaper J . A unique cell-surface protein phenotype distinguishes human small-cell from non-small-cell lung cancer. Proc Natl Acad Sci U S A. 1982; 79(15):4650-4. PMC: 346733. DOI: 10.1073/pnas.79.15.4650. View