Cigarette Smoke Exposure to Pig Larynx in an Inhalation Chamber

Overview

Journal J Voice

Specialty Otorhinolaryngology

Date 2018 Jul 10

PMID 29983221

Citations 6

Authors

Xinxin Liu

Allison Mustonen

Wei Zheng

M Preeti Sivasankar

Abigail C Durkes

Affiliations

Soon will be listed here.

Abstract

Objectives: This study investigated the effects of cigarette smoke exposure on the pig larynx using an inhalation chamber. Specifically, we compared the effects of cigarette smoke exposure from either 3 cigarettes per day (3cd) or 15 cigarettes per day (15cd) for 20 days.

Study Design: In vivo prospective design.

Methods: Female pigs were exposed via an inhalation chamber to cigarette smoke (3R4F research cigarettes) from 3cd (n = 6) or 15cd (n = 6) for 20 days. Outcomes included histopathology of vocal fold and airway tissues; gene expression of interleukins, TNF-α, and VEGF; protein levels of TNF-α and IL-6; and number of coughs recorded in the chamber.

Results: Pigs exposed to cigarette smoke from 15cd exhibited mild vocal fold edema as compared to the 3cd group on histopathological evaluation. There was also minimal inflammation of nasal and tracheal tissue characterized by presence of more granulocytes in the 15cd group compared to the 3cd group. Cough frequency was significantly greater for the 15cd group compared to the 3cd group.

Conclusions: A custom-designed large animal inhalation chamber successfully challenged pigs repeatedly, to varying levels of cigarette smoke. Future studies will combine such low levels of smoke exposure with other common challenges such as acid reflux to understand the multifactorial causation of laryngeal pathologies.

Citing Articles

Digital Vocal Biomarker of Smoking Status Using Ecological Audio Recordings: Results from the Colive Voice Study.

Ayadi H, Elbeji A, Despotovic V, Fagherazzi G Digit Biomark. 2024; 8(1):159-170.

PMID: 39473806 PMC: 11521430. DOI: 10.1159/000540327.

Modulation of mouse laryngeal inflammatory and immune cell responses by low and high doses of mainstream cigarette smoke.

Easwaran M, Martinez J, Kim J, Erickson-DiRenzo E Sci Rep. 2022; 12(1):18667.

PMID: 36333510 PMC: 9636197. DOI: 10.1038/s41598-022-23359-7.

Eliciting and Characterizing Porcine Vocalizations: When Pigs Fly.

Zhang L, Fujiki R, Brookes S, Calcagno H, Awonusi O, Kluender K J Voice. 2022; 38(5):993-1001.

PMID: 35504794 PMC: 9617810. DOI: 10.1016/j.jvoice.2022.02.023.

Short-term whole body cigarette smoke exposure induces regional differences in cellular response in the mouse larynx.

Easwaran M, Martinez J, Ramirez D, Gall P, Erickson-DiRenzo E Toxicol Rep. 2021; 8:920-937.

PMID: 33996505 PMC: 8099918. DOI: 10.1016/j.toxrep.2021.04.007.

Exploring the Pathophysiology of Reinke's Edema: The Cellular Impact of Cigarette Smoke and Vibration.

Grossmann T, Steffan B, Kirsch A, Grill M, Gerstenberger C, Gugatschka M Laryngoscope. 2020; 131(2):E547-E554.

PMID: 32569447 PMC: 7818424. DOI: 10.1002/lary.28855.

References

Marcotullio D, Magliulo G, Pezone T . Reinke's edema and risk factors: clinical and histopathologic aspects. Am J Otolaryngol. 2002; 23(2):81-4. DOI: 10.1053/ajot.2002.30961. View

Martins T, Campos K, da Silva Araujo N, Machado D, Bezerra F . Extrapulmonary effects of temporal exposure to cigarette smoke. Toxicol Ind Health. 2017; 33(9):717-725. DOI: 10.1177/0748233717715187. View

Rousseau B, Tateya I, Lim X, Munoz-Del-Rio A, Bless D . Investigation of anti-hyaluronidase treatment on vocal fold wound healing. J Voice. 2005; 20(3):443-51. DOI: 10.1016/j.jvoice.2005.06.002. View

Woodson G . Developing a porcine model for study of vocal fold scar. J Voice. 2012; 26(6):706-10. DOI: 10.1016/j.jvoice.2012.03.003. View

Gonzalez J, Carpi A . Early effects of smoking on the voice: a multidimensional study. Med Sci Monit. 2004; 10(12):CR649-56. View

Zaquia Leao H, Zettler C, Cambruzzi E, Lammers M, Rigon da Luz Soster P, Mello F . The Effects of Passive Smoking on Laryngeal and Tracheal Mucosa in Male Wistar Rats During Growth: An Experimental Study. J Voice. 2016; 31(1):126.e19-126.e24. DOI: 10.1016/j.jvoice.2015.12.014. View

Gately M, Renzetti L, Magram J, Stern A, Adorini L, Gubler U . The interleukin-12/interleukin-12-receptor system: role in normal and pathologic immune responses. Annu Rev Immunol. 1998; 16:495-521. DOI: 10.1146/annurev.immunol.16.1.495. View

Jiang J, Raviv J, Hanson D . Comparison of the phonation-related structures among pig, dog, white-tailed deer, and human larynges. Ann Otol Rhinol Laryngol. 2002; 110(12):1120-5. DOI: 10.1177/000348940111001207. View

Durkes A, Sivasankar M . In vivo investigation of acidified pepsin exposure to porcine vocal fold epithelia. Laryngoscope. 2015; 126(1):E12-7. PMC: 4705005. DOI: 10.1002/lary.25478. View

10.

Branski R, Saltman B, Sulica L, Szeto H, Duflo S, Felsen D . Cigarette smoke and reactive oxygen species metabolism: implications for the pathophysiology of Reinke's edema. Laryngoscope. 2009; 119(10):2014-8. DOI: 10.1002/lary.20592. View

11.

Nakamura M, Wada H, Honda K, Nakamoto K, Inui T, Sada M . Clarithromycin ameliorates pulmonary inflammation induced by short term cigarette smoke exposure in mice. Pulm Pharmacol Ther. 2015; 35:60-6. DOI: 10.1016/j.pupt.2015.09.005. View

12.

Stevens K, Thomson S, Jette M, Thibeault S . Quantification of Porcine Vocal Fold Geometry. J Voice. 2015; 30(4):416-26. PMC: 4757522. DOI: 10.1016/j.jvoice.2015.06.009. View

13.

Liu X, Zheng W, Sivasankar M . Acute Acrolein Exposure Induces Impairment of Vocal Fold Epithelial Barrier Function. PLoS One. 2016; 11(9):e0163237. PMC: 5028054. DOI: 10.1371/journal.pone.0163237. View

14.

Gaafar H . The effect of cigarette smoke on the vocal cord mucosa of the rabbit: An electron microscopic study. J Laryngol Otol. 1981; 95(7):721-9. DOI: 10.1017/s0022215100091349. View

15.

Wang J, Fang R, Peterson A, Jiang J . The Protective Role of Autophagy in Human Vocal Fold Fibroblasts under Cigarette Smoke Extract Exposure: A New Insight into the Study of Reinke's Edema. ORL J Otorhinolaryngol Relat Spec. 2015; 78(1):26-35. DOI: 10.1159/000442022. View

16.

Kuschner W, DAlessandro A, Wong H, Blanc P . Dose-dependent cigarette smoking-related inflammatory responses in healthy adults. Eur Respir J. 1996; 9(10):1989-94. DOI: 10.1183/09031936.96.09101989. View

17.

Rozen S, Skaletsky H . Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 1999; 132:365-86. DOI: 10.1385/1-59259-192-2:365. View

18.

Shiels M, Katki H, Freedman N, Purdue M, Wentzensen N, Trabert B . Cigarette smoking and variations in systemic immune and inflammation markers. J Natl Cancer Inst. 2014; 106(11). PMC: 4200029. DOI: 10.1093/jnci/dju294. View

19.

Chen H, Vlahos R, Bozinovski S, Jones J, Anderson G, Morris M . Effect of short-term cigarette smoke exposure on body weight, appetite and brain neuropeptide Y in mice. Neuropsychopharmacology. 2004; 30(4):713-9. DOI: 10.1038/sj.npp.1300597. View

20.

Carter C, Misra M, Maronpot R . Tracheal Morphologic and Protein Alterations FollowingShort-Term Cigarette Mainstream Smoke Exposure to Rats. J Toxicol Pathol. 2012; 25(3):201-7. PMC: 3434335. DOI: 10.1293/tox.25.201. View