Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2018 Jul 7

PMID 29978148

Citations 3

Authors

Benjamin K Liebov

Alejandro D Arroyo

Natalia I Rubtsova

Sofya A Osharovich

E James Delikatny

Anatoliy V Popov

Affiliations

Soon will be listed here.

Abstract

The near-infrared fluorescent activatable smart probe Pyro-phosphatidylethanolamine (PtdEtn)-QSY was synthesized and observed to selectively fluoresce in the presence of phosphatidylcholine-specific phospholipase C (PC-PLC). PC-PLC is an important biological target as it is known to be upregulated in a variety of cancers, including triple negative breast cancer. Pyro-PtdEtn-QSY features a QSY21 quenching moiety instead of the Black Hole Quencher-3 (BHQ-3) used previously because the latter contains an azo bond, which could lead to biological instability.

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