Hormonal Regulation of Oviductal Glycoprotein 1 (OVGP1; MUC9) in the Rhesus Macaque Cervix

Overview

Journal J Med Primatol

Publisher Wiley

Date 2018 Jul 5

PMID 29971796

Citations 8

Authors

Ov Daniel Slayden

Francis Kathryn E Friason

Kise Rosen Bond

Emily Cathleen Mishler

Affiliations

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Abstract

Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action.

Materials: Rhesus macaques were treated with estradiol (E ), E plus progesterone (P), and E plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry.

Results: OVGP1 transcript was maximal after E and reduced after treatment with E + P (P < .05). LNG also reduced OVGP1 expression (P < .05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples.

Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.

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References

Tepper N, Whiteman M, Marchbanks P, James A, Curtis K . Progestin-only contraception and thromboembolism: A systematic review. Contraception. 2016; 94(6):678-700. PMC: 11034842. DOI: 10.1016/j.contraception.2016.04.014. View

Saccary L, She Y, Oko R, Kan F . Hamster oviductin regulates tyrosine phosphorylation of sperm proteins during in vitro capacitation. Biol Reprod. 2013; 89(2):38. DOI: 10.1095/biolreprod.113.109314. View

Almeida-Francia C, Keator C, Mah K, Holden L, Hergert C, Slayden O . Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque. Hum Reprod. 2012; 27(6):1723-34. PMC: 3357194. DOI: 10.1093/humrep/des086. View

FERRARI A, Hafez E, SYNER F . Detection of ovulation by color changes in cervical mucus of crab-eating macaque (Macaca fascicularis). Int J Fertil. 1972; 17(4):169-77. View

Hendrix E, Hewetson A, Mansharamani M, Chilton B . Oviductin (Muc9) is expressed in rabbit endocervix. Endocrinology. 2001; 142(5):2151. DOI: 10.1210/endo.142.5.8285. View

Endrikat J, Gerlinger C, Richard S, Rosenbaum P, Dusterberg B . Ovulation inhibition doses of progestins: a systematic review of the available literature and of marketed preparations worldwide. Contraception. 2011; 84(6):549-57. DOI: 10.1016/j.contraception.2011.04.009. View

INSLER V . The evaluation and treatment of cervical mucus diseases leading to infertility. Adv Exp Med Biol. 1977; 89:477-88. DOI: 10.1007/978-1-4613-4172-7_32. View

Buhi W . Characterization and biological roles of oviduct-specific, oestrogen-dependent glycoprotein. Reproduction. 2002; 123(3):355-62. DOI: 10.1530/rep.0.1230355. View

Slayden O, Brenner R . A critical period of progesterone withdrawal precedes menstruation in macaques. Reprod Biol Endocrinol. 2006; 4 Suppl 1:S6. PMC: 1775066. DOI: 10.1186/1477-7827-4-S1-S6. View

10.

INSLER V, Glezerman M, Bernstein D . Diagnosis and treatment of the cervical factor of infertility. Reproduccion. 1981; 5(4):295-300. View

11.

Leader A, Wiseman D, Taylor P . The prediction of ovulation: a comparison of the basal body temperature graph, cervical mucus score, and real-time pelvic ultrasonography. Fertil Steril. 1985; 43(3):385-8. DOI: 10.1016/s0015-0282(16)48436-0. View

12.

Wright J, Pejovic T, Jurevic L, Bishop C, Hobbs T, Stouffer R . Ovarian surface epitheliectomy in the non-human primate: continued cyclic ovarian function and limited epithelial replacement. Hum Reprod. 2011; 26(6):1422-30. PMC: 3096558. DOI: 10.1093/humrep/der061. View

13.

Kanagawa H, Hafez E, Mori J, Kurosawa T, Kothari L . Cyclic changes in cervical mucus and LH levels in the bonnet macaque (Macaca radiata). Folia Primatol (Basel). 1973; 19(2):208-17. DOI: 10.1159/000155539. View

14.

Slayden O, Brenner R . Hormonal regulation and localization of estrogen, progestin and androgen receptors in the endometrium of nonhuman primates: effects of progesterone receptor antagonists. Arch Histol Cytol. 2005; 67(5):393-409. DOI: 10.1679/aohc.67.393. View

15.

Slayden O . Translational In Vivo Models for Women's Health: The Nonhuman Primate Endometrium--A Predictive Model for Assessing Steroid Receptor Modulators. Handb Exp Pharmacol. 2016; 232:191-202. DOI: 10.1007/164_2015_22. View

16.

Yang X, Zhao Y, Yang X, Kan F . Recombinant hamster oviductin is biologically active and exerts positive effects on sperm functions and sperm-oocyte binding. PLoS One. 2015; 10(4):e0123003. PMC: 4388664. DOI: 10.1371/journal.pone.0123003. View

17.

Singh S, Prasad S, Gupta H, Singhal S, Gupta A, Kumar A . Isolation and characterization of oviduct-specific glycoproteins from ampulla and isthmus parts of cyclic and acyclic buffalo for studying differential microenvironment. Appl Biochem Biotechnol. 2012; 166(7):1814-30. DOI: 10.1007/s12010-012-9599-6. View

18.

Lewis R, Taylor D, Natavio M, Melamed A, Felix J, Mishell Jr D . Effects of the levonorgestrel-releasing intrauterine system on cervical mucus quality and sperm penetrability. Contraception. 2010; 82(6):491-6. DOI: 10.1016/j.contraception.2010.06.006. View

19.

Martus N, Verhage H, Mavrogianis P, Thibodeaux J . Enhancement of bovine oocyte fertilization in vitro with a bovine oviductal specific glycoprotein. J Reprod Fertil. 1998; 113(2):323-9. DOI: 10.1530/jrf.0.1130323. View

20.

Woo M, Gilks C, Verhage H, Longacre T, Leung P, Auersperg N . Oviductal glycoprotein, a new differentiation-based indicator present in early ovarian epithelial neoplasia and cortical inclusion cysts. Gynecol Oncol. 2004; 93(2):315-9. DOI: 10.1016/j.ygyno.2004.01.047. View