Identification of the Escherichia Coli RecN Gene Product As a Major SOS Protein

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Nov 1

PMID 2997124

Citations 21

Authors

P W Finch

P Chambers

P T Emmerson

Affiliations

Soon will be listed here.

Abstract

The recA+ lexA+-dependent induction of four Escherichia coli SOS proteins was readily observed by two-dimensional gel analysis. In addition to the 38-kilodalton (kDa) RecA protein, which was induced in the greatest amounts and was readily identified, three other proteins of 115, 62, and 12 kDa were seen. The 115-kDa protein is the product of the uvrA gene, which is required for nucleotide excision repair and has previously been shown to be induced in the SOS response. The 62-kDa protein, which was induced to high intracellular levels, is the product of recN, a gene required for recBC-independent recombination. The recA and recN genes were partially derepressed in a recBC sbcB genetic background, a phenomenon which might account for the recombination proficiency of such strains. The 12-kDa protein has yet to be identified.

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References

Templin A, Kushner S, Clark A . Genetic analysis of mutations indirectly suppressing recB and recC mutations. Genetics. 1972; 72(2):105-15. PMC: 1212821. View

Picksley S, Lloyd R, BUCKMAN C . Genetic analysis and regulation of inducible recombination in Escherichia coli K-12. Cold Spring Harb Symp Quant Biol. 1984; 49:469-74. DOI: 10.1101/sqb.1984.049.01.053. View

Weiner J, BERTSCH L, Kornberg A . The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication. J Biol Chem. 1975; 250(6):1972-80. View

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

Hallewell R, Sherratt D . Isolation and characterization of Co1E2 plasmid mutants unable to kill colicin-sensitive cells. Mol Gen Genet. 1976; 146(3):239-45. DOI: 10.1007/BF00701246. View

Emmerson P, West S . Identification of protein X of Escherichia coli as the recA+/tif+ gene product. Mol Gen Genet. 1977; 155(1):77-85. DOI: 10.1007/BF00268563. View

McEntee K . Protein X is the product of the recA gene of Escherichia coli. Proc Natl Acad Sci U S A. 1977; 74(12):5275-9. PMC: 431681. DOI: 10.1073/pnas.74.12.5275. View

Gudas L, Mount D . Identification of the recA (tif) gene product of Escherichia coli. Proc Natl Acad Sci U S A. 1977; 74(12):5280-4. PMC: 431684. DOI: 10.1073/pnas.74.12.5280. View

Casadaban M, Cohen S . Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979; 76(9):4530-3. PMC: 411611. DOI: 10.1073/pnas.76.9.4530. View

10.

Yamamori T, Yura T . Temperature-induced synthesis of specific proteins in Escherichia coli: evidence for transcriptional control. J Bacteriol. 1980; 142(3):843-51. PMC: 294109. DOI: 10.1128/jb.142.3.843-851.1980. View

11.

Phillips T, Bloch P, Neidhardt F . Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins. J Bacteriol. 1980; 144(3):1024-33. PMC: 294767. DOI: 10.1128/jb.144.3.1024-1033.1980. View

12.

Irbe R, Oishi M . Prophage induction in a permeabilized cell system: induction by deoxyribonucleases and the role of recBC-deoxyribonuclease. J Bacteriol. 1980; 144(3):1061-7. PMC: 294771. DOI: 10.1128/jb.144.3.1061-1067.1980. View

13.

Kenyon C, Walker G . Expression of the E. coli uvrA gene is inducible. Nature. 1981; 289(5800):808-10. DOI: 10.1038/289808a0. View

14.

MILLER H, NASH H . Direct role of the himA gene product in phage lambda integration. Nature. 1981; 290(5806):523-6. DOI: 10.1038/290523a0. View

15.

Neidhardt F, VanBogelen R . Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem Biophys Res Commun. 1981; 100(2):894-900. DOI: 10.1016/s0006-291x(81)80257-4. View

16.

Sancar A, Williams K, Chase J, RUPP W . Sequences of the ssb gene and protein. Proc Natl Acad Sci U S A. 1981; 78(7):4274-8. PMC: 319772. DOI: 10.1073/pnas.78.7.4274. View

17.

Sancar A, Wharton R, Seltzer S, Kacinski B, Clarke N, RUPP W . Identification of the uvrA gene product. J Mol Biol. 1981; 148(1):45-62. DOI: 10.1016/0022-2836(81)90234-5. View

18.

Sancar A, Clarke N, Griswold J, Kennedy W, RUPP W . Identification of the uvrB gene product. J Mol Biol. 1981; 148(1):63-76. DOI: 10.1016/0022-2836(81)90235-7. View

19.

MILLER H, Kirk M, Echols H . SOS induction and autoregulation of the himA gene for site-specific recombination in Escherichia coli. Proc Natl Acad Sci U S A. 1981; 78(11):6754-8. PMC: 349128. DOI: 10.1073/pnas.78.11.6754. View

20.

Karu A, Belk E . Induction of E. coli recA protein via recBC and alternate pathways: quantitation by enzyme-linked immunosorbent assay (ELISA). Mol Gen Genet. 1982; 185(2):275-82. DOI: 10.1007/BF00330798. View