Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Neurocysticercosis

Overview

Journal Front Neurol

Specialty Neurology

Date 2018 Jul 5

PMID 29971042

Citations 22

Authors

Siyuan Fan

Xiaodong Qiao

Lei Liu

Honglong Wu

Jiali Zhou

Ruixue Sun

Qing Chen

Yan Huang

Chenhui Mao

Jing Yuan

Qiang Lu

Ying Ge

Yongjun Li

Haitao Ren

Jiawei Wang

Liying Cui

Weili Zhao

Hongzhi Guan

Affiliations

Soon will be listed here.

Abstract

Neurocysticercosis (NCC) is the most common helminthic infection of the central nervous system (CNS). The diagnosis of NCC is sometimes challenging due to its heterogenous clinical manifestations and the variable sensitivity and specificity of neuroimaging and serological tests. Next-generation sequencing (NGS) of cerebrospinal fluid (CSF) was used to detect pathogens in patients with clinically suspected CNS infections. A series of patients diagnosed with NCC is reviewed here. Using NGS of CSF, four patients were diagnosed with NCC. The reads corresponding to ranged from 478 to 117,362, with genomic coverage of 0.0564-11.15%. Reads corresponding to were not found in non-template controls and far exceeded those of the background microorganisms in patients with NCC, facilitating the interpretation of the NGS results. This case series demonstrates that NGS of CSF is promising in the diagnosis of NCC in difficult to diagnose cases. Larger studies are needed in the future.

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