Genome-wide Association Analysis of Intra-specific QTL Associated with the Resistance for Enteric Septicemia of Catfish

Overview

Journal Mol Genet Genomics

Specialty Genetics

Date 2018 Jul 4

PMID 29967962

Citations 9

Authors

Huitong Shi

Tao Zhou

Xiaozhu Wang

Yujia Yang

Chenglong Wu

Shikai Liu

Lisui Bao

Ning Li

Zihao Yuan

Yulin Jin

Suxu Tan

Wenwen Wang

Xiaoxiao Zhong

Guyu Qin

Xin Geng

Dongya Gao

Rex Dunham

Zhanjiang Liu

Affiliations

Soon will be listed here.

Abstract

Disease resistance is one of the most important traits for aquaculture industry. For catfish industry, enteric septicemia of catfish (ESC), caused by the bacterial pathogen Edwardsiella ictaluri, is the most severe disease, causing enormous economic losses every year. In this study, we used three channel catfish families with 900 individuals (300 fish per family) and the 690K catfish SNP array, and conducted a genome-wide association study to detect the quantitative trait loci (QTL) associated with ESC resistance. Three significant QTL, with two of located on LG1 and one on LG26, and three suggestive QTL located on LG1, LG3, and LG21, respectively, were identified to be associated with ESC resistance. With a well-assembled- and -annotated reference genome sequence, genes around the involved QTL regions were identified. Among these genes, 37 genes had known functions in immunity, which may be involved in ESC resistance. Notably, nlrc3 and nlrp12 identified here were also found in QTL regions of ESC resistance in the channel catfish × blue catfish interspecific hybrid system, suggesting this QTL was operating within both intra-specific channel catfish populations and interspecific hybrid backcross populations. Many of the genes of the Class I MHC pathway, for mediated antigen processing and presentation, were found in the QTL regions. The positional correlation found in this study and the expressional correlation found in previous studies indicated that Class I MHC pathway was significantly associated with ESC resistance. This study validated one QTL previously identified using the second and fourth generation of the interspecific hybrid backcross progenies, and identified five additional QTL among channel catfish families. Taken together, it appears that there are only a few major QTL for ESC disease resistance, making marker-assisted selection an effective approach for genetic improvements of ESC resistance.

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